Kamamoto T, Ohkubo T, Sakoda H, Taniguchi Y, Kita K, Yoshida Y, Uchino H
Jpn J Clin Oncol. 1986 Jun;16(2):107-15. doi: 10.1093/oxfordjournals.jjco.a039125.
Two new Philadelphia (Ph1) chromosome-positive cell lines, designated KPB-M8 and KPB-M15, were established from the peripheral blood of two patients with chronic myelogenous leukemia in blastic crisis. Both cell lines were characterized by blastic appearance, the presence of acid phosphatase activity, Fc gamma-receptor, and C3-receptor, and reactivity to monoclonal antibodies such as OKM1, MCS2, MY906, MY4 and MY7. These results indicate that KPB-M8 and KPB-M15 cells are of an undifferentiated blast nature. Both cells retained Ph1 chromosome, and showed numerical and structural changes upon chromosomal analysis. These cell lines should provide a useful source for studying differentiation of hemopoietic cells.
从两名处于原始细胞危象的慢性粒细胞白血病患者的外周血中建立了两个新的费城（Ph1）染色体阳性细胞系，分别命名为KPB-M8和KPB-M15。两个细胞系均具有原始细胞外观、酸性磷酸酶活性、Fcγ受体和C3受体，并且对OKM1、MCS2、MY906、MY4和MY7等单克隆抗体有反应。这些结果表明KPB-M8和KPB-M15细胞具有未分化的原始细胞性质。两种细胞均保留了Ph1染色体，并且在染色体分析时显示出数量和结构变化。这些细胞系应为研究造血细胞的分化提供有用的来源。
