Department of Energy and Materials Engineering, Dongguk University, 30 Pildong-ro 1-gil, Seoul, 04620, Republic of Korea.
Department of Nanoscience and Technology, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India.
Mikrochim Acta. 2021 Oct 6;188(11):365. doi: 10.1007/s00604-021-05022-6.
An ultrasensitive electrochemical immunosensor has been prepared using an immunofunctionalized zirconium (Zr)-based metal-organic framework (MOF) with gold (Au) decoration Au@UiO-66(NH) composite-coated glassy carbon electrode (GCE) for the determination of infectious hepatitis B surface antigen (HBsAg). We fabricated GCE with specific composite via immune-functionalization using anti-HBsAg with Au nanoparticles embedded in UiO-66(NH). The electrochemical sensing performance of the immunofunctionalized Au@UiO-66(NH)/GCE with HBsAg was characterized by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, there was a linear dynamic relationship in the buffer system between the electrical signal and HBsAg levels over the range 1.13 fg mL-100 ng mL (R = 0.999) with a detection limit of 1.13 fg mL. The total analysis time was 15 min per sample. Further validations were performed with HBsAg-spiked human serum samples, and similar detection limits as in the buffer system were observed with reduced signal intensities at lower concentrations of HBsAg (1, 10, and 100 fg mL) and minimal interference. The HBsAg electrochemical immunosensing assay had good selectivity and excellent reproducibility, thereby indicating its significant potential in the super-fast diagnosis of hepatitis B.
一种超灵敏的电化学免疫传感器已经制备出来，该传感器使用免疫功能化的基于锆的金属有机骨架（MOF）与金（Au）修饰的 Au@UiO-66（NH）复合涂层玻碳电极（GCE），用于测定传染性乙型肝炎表面抗原（HBsAg）。我们通过使用嵌入 UiO-66（NH）中的抗 HBsAg 进行免疫功能化，在 GCE 上制造了具有特定复合结构的传感器。Au@UiO-66（NH）/GCE 与 HBsAg 的免疫功能化的电化学传感性能通过循环伏安法和差分脉冲伏安法进行了表征。在优化条件下，在缓冲体系中，电信号与 HBsAg 水平之间存在线性动态关系，线性范围为 1.13 fg mL-100 ng mL（R = 0.999），检测限为 1.13 fg mL。每个样品的总分析时间为 15 分钟。进一步使用 HBsAg 加标人血清样品进行验证，在较低浓度的 HBsAg（1、10 和 100 fg mL）下观察到与缓冲体系相似的检测限，但信号强度降低，并且干扰最小。HBsAg 电化学免疫传感器具有良好的选择性和出色的重现性，表明其在乙型肝炎的超快速诊断中有很大的应用潜力。
