Henan Key Laboratory of Polyoxometalate Chemistry, Henan Joint International Research Laboratory of Environmental Pollution Control Materials, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 47504, PR China.
Food Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen, Guangdong, PR China.
Anal Chim Acta. 2020 Oct 9;1133:119-127. doi: 10.1016/j.aca.2020.07.080. Epub 2020 Aug 12.
Carcinoembryonic antigen (CEA) is a well-known cancer biomarker for the detection of several malignancies. The development of ultrasensitive CEA diagnostic tools is crucial for early detection and progression observation of tumors. Herein, a dual signal amplified sandwich-type electrochemical immunoassay was developed based on dual-labeled mesoporous silica nanospheres as a signal amplifier, combined with NiO@Au decorated graphene as a conductive layer for ultrasensitive and rapid determination of CEA. The dual-labeled mesoporous silica (DLMS) nanosphere, which was synthesized by entrapping Au nanorod (Au NR) and horseradish peroxidase (HRP) in the channels of amine-functionalized SBA-15 followed by subordinate antibody (Ab) conjugation which was denoted as Au NR@SBA-15/Ab-HRP. The dual signal amplification from Au NR@SBA-15 and HRP enhanced the sensitivity of the proposed immunoassay. Consequently, the developed DLMS based immunosensor displayed ultra-low limits of detection of 5.25 fg/mL and a wide range of linearity (0.1-5 pg/mL), which was extended for CEA determination in real-time samples with improved recoveries of >98%. Therefore, this dual amplification prototype would cater to the clinical requirements for the ultrasensitive detection of CEA biomarkers.
癌胚抗原（CEA）是一种用于检测多种恶性肿瘤的知名癌症生物标志物。开发超灵敏的 CEA 诊断工具对于肿瘤的早期检测和进展观察至关重要。在此，我们开发了一种基于双标记介孔硅纳米球作为信号放大器的双信号放大夹心型电化学免疫分析方法，结合 NiO@Au 修饰的石墨烯作为导电层，用于超灵敏和快速测定 CEA。双标记介孔硅（DLMS）纳米球是通过在胺功能化 SBA-15 的孔道内包埋金纳米棒（Au NR）和辣根过氧化物酶（HRP），然后进行次级抗体（Ab）缀合来合成的，该纳米球被标记为 Au NR@SBA-15/Ab-HRP。Au NR@SBA-15 和 HRP 的双信号放大增强了所提出免疫分析的灵敏度。因此，基于开发的 DLMS 的免疫传感器显示出超低的检测限为 5.25 fg/mL 和较宽的线性范围（0.1-5 pg/mL），可用于实时样品中 CEA 的测定，回收率提高到>98%。因此，这种双扩增原型将满足对超灵敏检测 CEA 生物标志物的临床需求。
