CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, 430074, Wuhan, China.
Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, 430074, Wuhan, China.
BMC Plant Biol. 2021 Oct 6;21(1):450. doi: 10.1186/s12870-021-03185-1.
Methyl jasmonate (MeJA), which has been identified as a lipid-derived stress hormone, mediates plant resistance to biotic/abiotic stress. Understanding MeJA-induced plant defense provides insight into how they responding to environmental stimuli.
In this work, the dynamic network analysis method was used to quantitatively identify the tipping point of growth-to-defense transition and detect the associated genes. As a result, 146 genes were detected as dynamic network biomarker (DNB) members and the critical defense transition was identified based on dense time-series RNA-seq data of MeJA-treated Arabidopsis thaliana. The GO functional analysis showed that these DNB genes were significantly enriched in defense terms. The network analysis between DNB genes and differentially expressed genes showed that the hub genes including SYP121, SYP122, WRKY33 and MPK11 play a vital role in plant growth-to-defense transition.
Based on the dynamic network analysis of MeJA-induced plant resistance, we provide an important guideline for understanding the growth-to-defense transition of plants' response to environment stimuli. This study also provides a database with the key genes of plant defense induced by MeJA.
茉莉酸甲酯（MeJA）作为一种脂类衍生的应激激素，介导植物对生物/非生物胁迫的抗性。了解 MeJA 诱导的植物防御机制可以深入了解它们如何对环境刺激做出反应。
在这项工作中，使用动态网络分析方法定量识别生长到防御的转变的临界点，并检测相关基因。结果，检测到 146 个基因作为动态网络生物标志物（DNB）成员，并基于拟南芥 MeJA 处理的密集时间序列 RNA-seq 数据确定了关键的防御转变点。GO 功能分析表明，这些 DNB 基因在防御相关术语中显著富集。DNB 基因与差异表达基因之间的网络分析表明，枢纽基因包括 SYP121、SYP122、WRKY33 和 MPK11，在植物生长到防御的转变中起着至关重要的作用。
基于 MeJA 诱导植物抗性的动态网络分析，为理解植物对环境刺激的生长到防御的转变提供了重要的指导。本研究还提供了一个包含 MeJA 诱导植物防御的关键基因的数据库。
