不同留茬高度和冻融胁迫对黑麦幼苗的生理效应。

Physiological effects of different stubble height and freeze-thaw stress on Secale cereale L. seedlings.

机构信息

Key Laboratory of Groundwater Resources and Environment of the Ministry of Education (Jilin University), Jilin Provincial Key Laboratory of Water Resources and Environment; College of New Energy and Environment, Jilin University, Changchun, 130012, China.

College of Plant Science, Jilin University, Changchun, 130062, China.

出版信息

BMC Plant Biol. 2021 Oct 6;21(1):451. doi: 10.1186/s12870-021-03235-8.

DOI:10.1186/s12870-021-03235-8

PMID:34615488

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8493682/

Abstract

BACKGROUND

As a biennial plant, Secale cereale L is usually harvested in the autumn in the northern part of China where the temperature difference between day and night is of great disparity Through the pot experiment, the seedlings were cut to 2, 6 and 10 cm stubble height, and the simulated freeze-thaw (FT) stress (10/- 5 °C) was carried out after 6 days regrowth. The physiological effects of FT with different stubble height were revealed by analyzing the relative water content (RWC), osmotic adjustment substance concentration (soluble sugar and protein), membrane peroxidation (MDA) and catalase (CAT) activity.

RESULTS

The results demonstrated that under freeze stress (- 5 °C), the content of soluble protein and MDA decreased and the seedlings of 2 cm treatment kept higher level of soluble protein and MDA, while the seedlings of 6 and 10 cm treatments kept higher level of the RWC, soluble sugar content, and CAT activity. After FT stress, the content of soluble sugar and protein, RWC in the 6 cm treatment were higher than those in 2 cm and 10 cm treatments, and the CAT activity in 10 cm treatment was the highest while the MDA content is lower.

CONCLUSION

These data suggest that keeping high stubble height is more adaptive for short-term FT stress.

摘要

背景

黑麦是一种两年生植物，在中国北方，通常在秋季收获，昼夜温差很大。通过盆栽实验，将幼苗剪至 2、6 和 10 cm 的茬高，在重新生长 6 天后进行模拟冻融（FT）胁迫（10/-5°C）。通过分析相对水含量（RWC）、渗透调节物质浓度（可溶性糖和蛋白质）、膜过氧化（MDA）和过氧化氢酶（CAT）活性，揭示了不同茬高 FT 的生理效应。

结果

结果表明，在冷冻胁迫（-5°C）下，可溶性蛋白和 MDA 的含量降低，2 cm 处理的幼苗保持较高水平的可溶性蛋白和 MDA，而 6 和 10 cm 处理的幼苗保持较高水平的 RWC、可溶性糖含量和 CAT 活性。在 FT 胁迫后，6 cm 处理的可溶性糖和蛋白质含量、RWC 高于 2 cm 和 10 cm 处理，10 cm 处理的 CAT 活性最高，MDA 含量最低。