Department of Animal Science, University of California, Davis , 2145 Meyer Hall, Davis, CA, 95616-8521, USA.
Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT, 05405-0148, USA.
J Mammary Gland Biol Neoplasia. 2021 Sep;26(3):263-276. doi: 10.1007/s10911-021-09499-y. Epub 2021 Oct 6.
We previously showed that dietary trans-10, cis-12 conjugated linoleic acid (10,12 CLA) stimulates estrogen-independent mammary growth in young ovariectomized mice. Here we investigated the effects of in utero or postnatal exposure to cis-9, trans-11 (9,11 CLA) and 10,12 CLA on postnatal development of the mammary gland and its responsiveness to ovarian steroids. In the first experiment we fed dams different CLA prior to and during gestation, then cross fostered female pups onto control fed dams prior to assessing the histomorphology of their mammary glands. Pregnant dams in the second experiment were similarly exposed to CLA, after which their female pups were ovariectomized then treated with 17β-estradiol (E), progesterone (P) or E + P for 5 days. In a third experiment, mature female mice were fed different CLA for 28 days prior to ovariectomy, then treated with E, P or E + P. Our data indicate that 10,12 CLA modifies the responsiveness of the mammary glands to E or E + P when exposure occurs either in utero, or postnatally. These findings underline the sensitivity of the mammary glands to dietary fatty acids and reinforce the potential for maternal nutrition to impact postnatal development of the mammary glands and their risk for developing cancer.
我们之前的研究表明，饮食中的反式-10，顺式-12 共轭亚油酸（10,12 CLA）可刺激年轻去卵巢小鼠的雌激素非依赖性乳腺生长。在这里，我们研究了宫内或产后暴露于顺式-9，反式-11（9,11 CLA）和 10,12 CLA 对乳腺产后发育及其对卵巢类固醇的反应性的影响。在第一个实验中，我们在妊娠前和妊娠期间给母鼠喂食不同的 CLA，然后将雌性幼鼠交叉寄养到喂食对照饲料的母鼠中，然后评估其乳腺的组织形态。在第二个实验中，妊娠母鼠也以类似的方式暴露于 CLA，之后对其雌性幼鼠进行卵巢切除术，然后用 17β-雌二醇（E）、孕酮（P）或 E+P 处理 5 天。在第三个实验中，成熟雌性小鼠在卵巢切除术前 28 天喂食不同的 CLA，然后用 E、P 或 E+P 处理。我们的数据表明，10,12 CLA 改变了乳腺对 E 或 E+P 的反应性，无论是在宫内还是产后暴露。这些发现强调了乳腺对膳食脂肪酸的敏感性，并增强了母体营养对乳腺产后发育及其患癌症风险的潜在影响。
