环状 RNA circCRKL 通过 miR-196a-5p/miR-196b-5p/p27 轴抑制急性髓系白血病细胞的增殖。
Circular RNA circCRKL inhibits the proliferation of acute myeloid leukemia cells via the miR-196a-5p/miR-196b-5p/p27 axis.
机构信息
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
出版信息
Bioengineered. 2021 Dec;12(1):7704-7713. doi: 10.1080/21655979.2021.1982310.
As a new type of non-coding RNA, the role of circular RNA (circRNA) in various diseases and tumors has received considerable attention. Studies have shown that circRNAs play an important role in the progression of acute myeloid leukemia (AML) via different mechanisms. However, the specific underlying molecular mechanism of circRNAs in the proliferation of AML cells remians unclear. This study aimed to clarify the biological role and mechanism of circCRKL in AML. The results indicated low circCRKL expression in AML cell lines and samples. Moreover, the overexpression of circCRKL inhibited the proliferation and colony-forming ability of AML cells, while its silencing promoted them. In addition, bioinformatics tools and luciferase assays revealed that circCRKL could sponge miR-196a-5p and miR-196b-5p to promote the expression of p27. Furthermore, circCRKL inhibited AML cell proliferation via the miR-196a-5p/miR-196b-5p/p27 axis, suggesting a potential new target for AML therapy.
环状 RNA（circRNA）作为一种新型的非编码 RNA，其在各种疾病和肿瘤中的作用引起了相当大的关注。研究表明，circRNAs 通过不同的机制在急性髓系白血病（AML）的进展中发挥重要作用。然而，circRNAs 在 AML 细胞增殖中的具体潜在分子机制尚不清楚。本研究旨在阐明 circCRKL 在 AML 中的生物学作用和机制。结果表明，AML 细胞系和样本中 circCRKL 的表达较低。此外，circCRKL 的过表达抑制了 AML 细胞的增殖和集落形成能力，而其沉默则促进了它们的增殖和集落形成能力。此外，生物信息学工具和荧光素酶报告基因实验表明，circCRKL 可以海绵吸附 miR-196a-5p 和 miR-196b-5p，从而促进 p27 的表达。此外，circCRKL 通过 miR-196a-5p/miR-196b-5p/p27 轴抑制 AML 细胞增殖，提示其可能成为 AML 治疗的新靶点。
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