Quintal-Martínez Juan Pablo, Quintal-Ortiz Irma Guadalupe, Alonzo-Salomón Ligia Gabriela, Muñoz-Rodríguez David, Segura-Campos Maira Rubi
Faculty of Chemical Engineering, Autonomous University of Yucatán, Merida, Yucatan, Mexico.
Hematology Laboratory, Dr. Hideyo Noguchi Regional Research Center, Merida, Yucatan, Mexico.
J Med Food. 2021 Dec;24(12):1304-1312. doi: 10.1089/jmf.2021.0008. Epub 2021 Oct 6.
In Mexico, (chaya) has been used to treat cardiovascular diseases (CVD). Because CVD are the number one cause of mortality, chaya use has become a health strategy. The aim of this study was to evaluate the antithrombotic activity and identify the metabolites in the most active extract. Aqueous (Aq), ethanolic (EtOH), acetonic (An), ethyl acetate (AcOEt), diethyl ether (EtO), and hexanic (Hx) extracts were obtained. Platelet aggregation, phospholipase A, prothrombin time (PT), activated partial thromboplastin time (aPTT), and clot lysis were evaluated. Metabolites were identified by gas chromatography-mass spectrometry (GC-MS). EtOH showed the greatest inhibition of platelet aggregation and phospholipase A. Ac had the greatest effect on PT and aPTT. AcOEt had the greatest effect on clot lysis. EtOH, with the highest potential, was analyzed by GC-MS; fatty acids and triterpenes were identified. Thus, EtOH showed greater antiplatelet activity and other extracts showed moderate activity. This is a preliminary antithrombotic study. Future research will allow the development of nutraceuticals or functional ingredients for the prevention and treatment of thrombosis.
在墨西哥，（柴亚）已被用于治疗心血管疾病（CVD）。由于心血管疾病是首要死因，使用柴亚已成为一项健康策略。本研究的目的是评估抗血栓活性并鉴定最具活性提取物中的代谢物。获得了水提取物（Aq）、乙醇提取物（EtOH）、丙酮提取物（An）、乙酸乙酯提取物（AcOEt）、二乙醚提取物（EtO）和己烷提取物（Hx）。评估了血小板聚集、磷脂酶A、凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）和血块溶解情况。通过气相色谱-质谱联用仪（GC-MS）鉴定代谢物。乙醇对血小板聚集和磷脂酶A的抑制作用最大。丙酮对凝血酶原时间和活化部分凝血活酶时间的影响最大。乙酸乙酯对血块溶解的影响最大。对具有最高活性的乙醇提取物进行了GC-MS分析；鉴定出了脂肪酸和三萜类化合物。因此，乙醇显示出更强的抗血小板活性，其他提取物显示出中等活性。这是一项初步的抗血栓研究。未来的研究将有助于开发用于预防和治疗血栓形成的营养保健品或功能性成分。
