Llombart-Bosch A, Blache R, Peydro-Olaya A
Cancer. 1978 Apr;41(4):1362-73. doi: 10.1002/1097-0142(197804)41:4<1362::aid-cncr2820410421>3.0.co;2-f.
The characteristics of 35 round cell sarcomas of bones are analyzed by optical and electron microscopical means, 28 cases of Ewing's sarcoma and 7 cases of malignant lymphomas (reticulum cell sarcoma) being distinguished. The existence of a morphological diversity within the Ewing's sarcoma group allows the authors to differentiate a conventional form of Ewing's sarcoma (21 cases) consisting of principal blastemic cells and secondary degenerative ones (dark cells) from an atypical variant (7 cases of atypical Ewin's sarcoma) whose structure adopts either an immature mesenchymal or histiocytic appearance. The ultrastructure of the atypical Ewing's cells demonstrates variability in size and in the nucleus, which is grooved and possesses prominent nucleoli. In the cytoplasm, in addition to the existence of a variable amount of glycogen, more highly differentiated structures appear when a comparison is made with the principal cells of the conventional Ewing's sarcoma. There exist filaments and mitochondria complexes, desmosomic-like junctions and profiles of endoplasmic reticulum. No transitional forms between the conventional and the atypical variants of Ewing's sarcoma have been observed. According to this analysis, when compared to other bone malignancies, the presence of glycogen as a means for identifying Ewing's sarcoma seems to be of restricted value. No clinical or anatomical differences could be established between either variant of Ewing's sarcoma.
通过光学显微镜和电子显微镜手段分析了35例骨圆形细胞肉瘤的特征，其中区分出28例尤因肉瘤和7例恶性淋巴瘤（网状细胞肉瘤）。尤因肉瘤组内存在形态多样性，这使作者能够将由主要母细胞和继发性退变细胞（暗细胞）组成的传统形式的尤因肉瘤（21例）与非典型变体（7例非典型尤因肉瘤）区分开来，非典型变体的结构呈现未成熟间充质或组织细胞外观。非典型尤因细胞的超微结构显示大小和细胞核存在变异性，细胞核有沟且核仁突出。在细胞质中，除了存在数量不等的糖原外，与传统尤因肉瘤的主要细胞相比，还出现了分化程度更高的结构。存在细丝和线粒体复合物、桥粒样连接以及内质网轮廓。未观察到尤因肉瘤传统变体和非典型变体之间的过渡形式。根据该分析，与其他骨恶性肿瘤相比，糖原作为识别尤因肉瘤的手段似乎价值有限。尤因肉瘤的两种变体在临床或解剖学上均未发现差异。
