与墨西哥北部异烟肼耐药结核分枝杆菌菌株相关的研究。
RDMycobacterium tuberculosis strains associated with isoniazid resistance in Northern Mexico.
机构信息
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Laboratorio Interacción ambiente-microorganismo, Reynosa, Tamaulipas, Mexico.
Universidad Autónoma de Nuevo León, Facultad de Medicina y Hospital Universitario Dr. José Eleuterio González, Departamento de Microbiología, Monterrey N.L., Mexico.
出版信息
Enferm Infecc Microbiol Clin (Engl Ed). 2021 Oct;39(8):399-402. doi: 10.1016/j.eimce.2020.07.010.
BACKGROUND
It has been established that the genomic background of Mycobacterium tuberculosis may influence disease progression, in particular for the Beijing family and the Latin American and Mediterranean (LAM)/RD strains. The purpose of this study was to evaluate the prevalence of the LAM/RD genotype in cases of tuberculosis from Mexico and their drug susceptibility profile.
METHODS
Two hundred eighteen M. tuberculosis isolates were screened by 43-spacer spoligotyping. The LAM/RD genotype was confirmed by multiplex PCR, and the drug susceptibility testing was carried out in solid Löwenstein-Jensen media.
RESULTS
Among the LAM strains identified, 24 (63.1%) were confirmed as M. tuberculosis RD. All RD strains shared the RD174 deletion, that was associated with isoniazid resistance (p=0.0264).
CONCLUSIONS
We documented for the first time the isolation of the LAM/RD genotype in pulmonary cases of tuberculosis in Mexico, and we found resistance to the first-line anti-tuberculosis drug isoniazid in these strains.
背景
已经证实，结核分枝杆菌的基因组背景可能会影响疾病的进展，特别是对于北京家族和拉丁美洲/地中海（LAM）/RD 菌株。本研究的目的是评估来自墨西哥的结核病病例中 LAM/RD 基因型的流行情况及其药敏谱。
方法
通过 43 间隔 spoligotyping 筛选了 218 株结核分枝杆菌分离株。通过多重 PCR 确认 LAM/RD 基因型，并在 Löwenstein-Jensen 固体培养基中进行药物敏感性测试。
结果
在所鉴定的 LAM 株中，有 24 株（63.1%）被确认为结核分枝杆菌 RD。所有 RD 株均携带 RD174 缺失，与异烟肼耐药相关（p=0.0264）。
结论
我们首次在墨西哥的肺结核病例中分离出 LAM/RD 基因型，并在这些菌株中发现了对一线抗结核药物异烟肼的耐药性。
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