Department of Animal Biology, School of Natural Sciences, University of Tabriz, 51555, Tabriz, Iran.
Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Kurdistan Region, Erbil, Iraq.
Mol Biol Rep. 2021 Nov;48(11):7215-7222. doi: 10.1007/s11033-021-06713-2. Epub 2021 Oct 8.
Curcumin is a polyphenolic natural compound, which has demonstrated to possess antioxidant, anti-inflammatory, and anticancer effects in vitro & in vivo. However, its applicability in cancer therapy has been limited due to its poor cellular uptake. Here, we aimed to evaluate the anticancer effect of novel gemini curcumin (Gemini-Cur) on the gastric cancer AGS cells.
The AGS cancerous and HFF-2 non-cancerous cells were treated with Gemini-Cur and curcumin (Cur) in a time- and dose-dependent manner. Cellular toxicity was studied using MTT, fluorescence microscopy, annexin V/FITC, and cell cycle assays. Additionally, real-time PCR and western blotting were employed to evaluate the expression of Bax, Bcl-2 and survivin genes.
Our data indicated that Gemini-Cur is significantly taken into AGS cells compared to Cur. Moreover, the viability of Gemini-Cur treated cells was significantly reduced in a time- and dose-dependent manner (p < 0.001). Gemini-Cur compound induced G2/M cell cycle arrest that was followed by apoptosis in a time-dependent manner (p < 0.0001).
Taken together, our findings support the idea that Gemini-Cur has the potential to be considered as an anticancer agent.
姜黄素是一种多酚类天然化合物，已被证明具有体外和体内抗氧化、抗炎和抗癌作用。然而，由于其细胞摄取能力差，其在癌症治疗中的应用受到限制。在这里，我们旨在评估新型双姜黄素（Gemini-Cur）对胃癌 AGS 细胞的抗癌作用。
AGS 癌细胞和 HFF-2 非癌细胞以时间和剂量依赖的方式用 Gemini-Cur 和姜黄素（Cur）处理。使用 MTT、荧光显微镜、Annexin V/FITC 和细胞周期分析研究细胞毒性。此外，还使用实时 PCR 和 Western blot 评估 Bax、Bcl-2 和 survivin 基因的表达。
我们的数据表明，与姜黄素相比，Gemini-Cur 显著被 AGS 细胞摄取。此外，Gemini-Cur 处理的细胞活力呈时间和剂量依赖性显著降低（p<0.001）。Gemini-Cur 化合物诱导 G2/M 细胞周期停滞，随后呈时间依赖性凋亡（p<0.0001）。
综上所述，我们的研究结果支持 Gemini-Cur 有潜力被认为是一种抗癌药物的观点。
