双氢青蒿素通过诱导细胞凋亡抑制卵巢癌细胞 OVCAR-3 的增殖。
Gemini Curcumin Suppresses Proliferation of Ovarian Cancer OVCAR-3 Cells via Induction of Apoptosis.
机构信息
Department of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran.
Medical Research Center, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
出版信息
Anticancer Agents Med Chem. 2021;21(6):775-781. doi: 10.2174/1871520620666200807223340.
BACKGROUND
Ovarian cancer has the highest mortality rate among gynecological malignancies. Despite recent advances in treatment, most patients still suffer from poor prognosis. Curcumin has shown highly cytotoxic effects against different types of cancer. However, its poor bioavailability restricts its clinical application. Gemini Curcumin (Gemini-Cur) has been developed to overcome this limitation.
OBJECTIVE
Here, we aimed to unravel the inhibitory effect of Gemini-Cur in ovarian cancer.
METHODS
OVCAR-3 cells were treated with free curcumin and Gemni-Cur in a time- and dose-dependent manner. Then, the anticancer activity was investigated by uptake kinetics, cellular viability and apoptotic assays. Furthermore, we evaluated the BAX/Bcl-2 expression ratio by real-time PCR and western blotting.
RESULTS
Our data showed that gemini surfactant nanoparticles enhance the cellular uptake of curcumin compared to free curcumin (p<0.01). Regarding the growth inhibitory effect of nano-curcumin, the results demonstrated that Gemini-Cur suppresses the proliferation of OVCAR-3 cells through induction of apoptosis (p<0.001).
CONCLUSION
The results illustrate that Gemini-Cur nanoparticles have a great potential for developing novel therapeutics against ovarian cancer.
背景
卵巢癌是妇科恶性肿瘤中死亡率最高的一种。尽管最近在治疗方面取得了进展，但大多数患者的预后仍然较差。姜黄素对不同类型的癌症具有高度细胞毒性作用。然而，其较差的生物利用度限制了其临床应用。双姜黄素（Gemini-Cur）的开发就是为了克服这一限制。
目的
本研究旨在揭示 Gemini-Cur 对卵巢癌的抑制作用。
方法
用时间和剂量依赖性方式用游离姜黄素和 Gemini-Cur 处理 OVCAR-3 细胞。然后，通过摄取动力学、细胞活力和凋亡测定来研究抗癌活性。此外，通过实时 PCR 和 Western blot 评估 BAX/Bcl-2 表达比值。
结果
我们的数据表明，Gemini 表面活性剂纳米粒子比游离姜黄素（p<0.01）更能增强姜黄素的细胞摄取。关于纳米姜黄素的生长抑制作用，结果表明 Gemini-Cur 通过诱导细胞凋亡来抑制 OVCAR-3 细胞的增殖（p<0.001）。
结论
结果表明，Gemini-Cur 纳米粒子在开发针对卵巢癌的新型治疗方法方面具有巨大潜力。
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