Department of Bioinformatics, Graduate School of Engineering, Soka University, Hachioji, Tokyo, Japan.
Glycan & Life System Integration Center (GaLSIC), Soka University, Hachioji, Tokyo, Japan.
Methods Mol Biol. 2022;2303:675-685. doi: 10.1007/978-1-0716-1398-6_51.
3'-Phosphoadenosine 5'-phosphosulfate transporters (PAPSTs) play an important role in transporting 3'-phosphoadenosine 5'-phosphosulfate (PAPS), the universal sulfuryl donor for sulfation, from the cytosol into the lumen of the Golgi apparatus. Here, we describe three methods for the analysis of PAPST; a transporter activity assay with yeast or mammalian cell fraction, real-time reverse transcription polymerase chain reaction on tissue samples, and immunohistochemistry on brain sections.
3'-磷酸腺苷 5'-磷酸硫酸转移酶（PAPSTs）在将 3'-磷酸腺苷 5'-磷酸硫酸（PAPS），即硫酸化的通用硫供体，从细胞质转运到高尔基器腔中方面发挥着重要作用。在这里，我们描述了三种分析 PAPST 的方法；酵母或哺乳动物细胞级分的转运体活性测定、组织样品的实时逆转录聚合酶链反应和脑切片的免疫组织化学。
