Carruthers J A, Ewins A R
Clin Exp Immunol. 1978 Jan;31(1):38-44.
The sera of seven out of eight patients with Herpes gestationis contained a factor which fixed C3 but not detectable amounts of immunoglobulin (Ig) on the basement membrane zone (BMZ) of normal human skin. Using C2-deficient human serum and C4-deficient guinea-pig serum, fixation was shown to be dependent upon activation of the classical pathway, and chromatography and immunoabsorption studies indicated the H. gestationis factor to be an IgG immunoglobulin. In all seven sera the H. gestationis factor bound to, and could be eluted from, the eluate of staphylococcal protein A. Although the H. gestationis factor failed to block staining of human sera by a FITC-conjugated antibody prepared from bullous pemphigoid (BP) serum, complement fixation by five out of seven sera of patients with H. gestationis was blocked using F(ab')2 of IgG prepared from pooled BP serum. In a single patient rapid resolution of lesions followed plasma exchange on two separate occasions, indicating the pathogenetic importance of humoral factors in this disease.
8例妊娠疱疹患者中有7例患者的血清含有一种能固定C3但在正常人皮肤基底膜区（BMZ）检测不到免疫球蛋白（Ig）量的因子。使用C2缺陷型人血清和C4缺陷型豚鼠血清，发现补体固定依赖于经典途径的激活，色谱分析和免疫吸附研究表明妊娠疱疹因子是一种IgG免疫球蛋白。在所有7份血清中，妊娠疱疹因子与葡萄球菌蛋白A洗脱液结合并可从其中洗脱。尽管妊娠疱疹因子未能阻断由大疱性类天疱疮（BP）血清制备的异硫氰酸荧光素（FITC）偶联抗体对人血清的染色，但使用从合并的BP血清制备的IgG的F（ab'）2可阻断7例妊娠疱疹患者中5例患者血清的补体固定。在1例患者中，在两次单独的血浆置换后皮损迅速消退，表明体液因子在该疾病发病机制中的重要性。
