在流行地区，开发并验证一种改良的酶联免疫吸附试验方法，用于检测来自干燥血斑（DBS）样本的 HEV IgG 抗体。
Developing and validating a modified enzyme linked immunosorbent assay method for detecting HEV IgG antibody from dried blood spot (DBS) samples in endemic settings.
机构信息
Institute for Developing Science and Health Initiatives (ideSHi), Mohakhali, Dhaka, Bangladesh; Department of Microbiology, University of Dhaka, Dhaka, Bangladesh; Department of Immunology, Bangladesh University of Health Sciences, Dhaka, Bangladesh.
Enteric and Respiratory Infections, Infectious Diseases Division, International Centre for Diarrheal Disease Research, Bangladesh (icddr,b), Bangladesh.
出版信息
Microbes Infect. 2022 Mar;24(2):104890. doi: 10.1016/j.micinf.2021.104890. Epub 2021 Oct 7.
Serological analysis is an integral part of laboratory practice nowadays. The present study was aimed to develop and validate a modified Enzyme linked Immunosorbent Assay (ELISA) for determination of IgG antibody against Hepatitis E Virus (HEV) using dried blood spots (DBS) and corresponding plasma samples. A total of 65 samples (45 HEV patients, 20 healthy controls) were analyzed. DBS and plasma samples demonstrated equivalent optical densities for detecting anti-HEV IgG. A highly significant correlation was observed between plasma and DBS sample absorbances (R = 0.98; p < 0.001) at dilution 1:200, indicating true agreement between the two procedures. The assay exhibited decent linearity and showed no effect of physiological hematocrit on assay performance. Data suggested recommendable promise in using DBS as a suitable alternative to plasma samples to determine HEV IgG antibody evidenced by significant correlation with plasma results. Therefore, identical method for processing DBS specimens including it's proper storage is recommended for implementation of a modified ELISA in different settings.
血清学分析是当今实验室实践的一个组成部分。本研究旨在开发和验证一种改良的酶联免疫吸附试验（ELISA），用于使用干血斑（DBS）和相应的血浆样本测定抗戊型肝炎病毒（HEV）的 IgG 抗体。共分析了 65 个样本（45 例 HEV 患者，20 例健康对照）。DBS 和血浆样本在检测抗-HEV IgG 方面表现出等效的光密度。在稀释度为 1:200 时，血浆和 DBS 样本吸光度之间观察到高度显著的相关性（R=0.98;p<0.001），表明两种方法之间存在真正的一致性。该测定法表现出良好的线性度，并且生理血细胞比容对测定性能没有影响。数据表明，使用 DBS 作为替代血浆样本测定 HEV IgG 抗体具有很好的应用前景，与血浆结果具有显著相关性。因此，建议采用相同的方法处理 DBS 标本，包括适当的储存，以在不同环境下实施改良的 ELISA。
Zotero 插件