Key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, China.
Department of Neonatology, National Clinical Research Centre for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.
EBioMedicine. 2021 Oct;72:103607. doi: 10.1016/j.ebiom.2021.103607. Epub 2021 Oct 7.
The initial clinical manifestations and abdominal imaging findings of neonates with necrotising enterocolitis (NEC) and food protein-induced enterocolitis syndrome (FPIES) are sometimes similar; however, their prognosis and therapies are different. We aimed to evaluate the utility of interleukin (IL)-27 as a differentiation marker between NEC and highly suspected early onset (HSEO)-FPIES.
All samples used in this study were obtained from the neonatal diagnosis centre of Children's Hospital of Chongqing Medical University. In the case-control study, neonates with NEC (n = 13), HSEO-FPIES (n = 9), and jaundice (control, n = 8) were enroled to determine the serum IL-27 levels using commercial enzyme-linked immunosorbent assay (ELISA) kits. In the validation cohort study, the NEC (n = 87), HSEO-FPIES (n = 62), and jaundice (control, n = 54) groups were included to analyse the diagnostic efficiency of IL-27 for discriminating between NEC and HSEO-FPIES using a receiver operating characteristic (ROC) curve.
In the case-control study, IL-27 levels were higher in the NEC group than in the HSEO-FPIES group (p = 0·005). In the cohort study, the area under the ROC curve (AUC) of IL-27 for differentiating NEC from HSEO-FPIES was 0·878, which was higher than the AUCs of IL-6 (0·761), C-reactive protein (0·800), white blood cell count (0·637), neutrophils (0·765), lymphocytes (0·782), neutrophil to lymphocyte ratio (0·781), and platelet count (0·729).
Serum IL-27 is a novel biomarker that may potentially discriminate NEC from HSEO-FPIES in neonates.
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坏死性小肠结肠炎（NEC）和食物蛋白诱导的小肠结肠炎综合征（FPIES）新生儿的初始临床表现和腹部影像学表现有时相似，但预后和治疗方法不同。我们旨在评估白细胞介素（IL）-27作为 NEC 和高度疑似早发（HSEO）-FPIES 之间鉴别标志物的效用。
本研究所有样本均来自重庆医科大学附属儿童医院新生儿诊断中心。在病例对照研究中，纳入 NEC（n=13）、HSEO-FPIES（n=9）和黄疸（对照组，n=8）患儿，采用商业酶联免疫吸附试验（ELISA）试剂盒测定血清 IL-27 水平。在验证队列研究中，纳入 NEC（n=87）、HSEO-FPIES（n=62）和黄疸（对照组，n=54）患儿，采用受试者工作特征（ROC）曲线分析 IL-27 区分 NEC 和 HSEO-FPIES 的诊断效率。
在病例对照研究中，NEC 组的 IL-27 水平高于 HSEO-FPIES 组（p=0.005）。在队列研究中，IL-27 区分 NEC 和 HSEO-FPIES 的 ROC 曲线下面积（AUC）为 0.878，高于 IL-6（0.761）、C 反应蛋白（0.800）、白细胞计数（0.637）、中性粒细胞（0.765）、淋巴细胞（0.782）、中性粒细胞与淋巴细胞比值（0.781）和血小板计数（0.729）的 AUC。
血清 IL-27 是一种新型生物标志物，可能有助于区分 NEC 和 HSEO-FPIES 新生儿。
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