Northeast Biotechnology Network, Graduate Program of Biotechnology, Campus do Itaperi, State University of Ceará, Fortaleza, Ceará, Brazil.
Laboratory of Biotechnology and Molecular Biology, Health Sciences Center (CCS), Itaperi Campus, State University of Ceará, Fortaleza, Ceará, Brazil.
J Biomol Struct Dyn. 2022;40(23):13062-13074. doi: 10.1080/07391102.2021.1981449. Epub 2021 Oct 10.
Sm. belongs to the family Turneraceae and is found in Brazil. The present study evaluated the antinociceptive, anti-inflammatory, and hypoglycemic potential of flower extract (EtFloTsu) in zebrafish (). The total phenol and flavonoid contents of EtFloTsu were determined and identified using the Folin Ciocalteu reagent and aluminum chloride (AlCl), respectively. The constituents of the extract were identified by HPLC-DAD, and the antioxidant activity (DPPH) was determined, toxicity in brine shrimp, and acute toxicity of 96 h in adult zebrafish. In addition, adult zebrafish (n = 6/fish) were treated orally with EtFloTsu (4, 20, or 40 mg/kg; vo) and subjected to formalin-induced nociception tests (with its possible mechanism of action with camphor), carrageenan-induced inflammation, and D-glucose-induced hyperglycemia (111 mM). Oxidative stress in the liver and brain tissues was assessed. EtFloTsu showed high levels of phenolic and flavonoid compounds with antioxidant activity. The phytochemicals chlorogenic acid, luteolin-7-o-glucoside, vitexin, and apigenin-7-o-glucoside were also identified in EtFloTsu. The synergism between these constituents was possibly responsible for the antinociceptive (via TRPA1), anti-inflammatory, and hypoglycemic effects of EtFloTsu in adult zebrafish, without causing toxicity in animals. Therefore, flowers have therapeutic agents that could treat pain, inflammation, diabetes, and related complications.Communicated by Ramaswamy H. Sarma.
小桐花属于梧桐科，分布于巴西。本研究评估了花提取物（EtFloTsu）在斑马鱼中的镇痛、抗炎和降血糖作用。采用福林-肖卡（Folin Ciocalteu）试剂和三氯化铝（AlCl）分别测定了 EtFloTsu 的总酚和总黄酮含量。采用高效液相色谱-二极管阵列检测（HPLC-DAD）法鉴定提取物的成分，并测定其抗氧化活性（DPPH）、对卤虫的毒性以及成年斑马鱼 96 小时的急性毒性。此外，成年斑马鱼（n = 6/鱼）经口给予 EtFloTsu（4、20 或 40 mg/kg；vo），并进行福尔马林诱导的疼痛测试（可能的作用机制为樟脑）、角叉菜胶诱导的炎症和 D-葡萄糖诱导的高血糖（111 mM）。评估了肝脏和脑组织中的氧化应激。EtFloTsu 表现出高含量的酚类和类黄酮化合物，具有抗氧化活性。EtFloTsu 中还鉴定出了绿原酸、木樨草素-7-O-葡萄糖苷、荭草苷和芹菜素-7-O-葡萄糖苷等植物化学物质。这些成分的协同作用可能是 EtFloTsu 在成年斑马鱼中发挥镇痛（通过 TRPA1）、抗炎和降血糖作用的原因，而不会对动物造成毒性。因此，小桐花具有治疗疼痛、炎症、糖尿病及相关并发症的药物潜力。
