Nian Lili, Zhang Xiaoning, Yi Xianfeng, Liu Xuelu, Ain Noor Ul, Yang Yingbo, Li Xiaodan, Haider Fasih Ullah, Zhu Xiaolin
College of Forestry, Gansu Agricultural University, Lanzhou, 730070 China.
The Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning, 530001 China.
Physiol Mol Biol Plants. 2021 Sep;27(9):1979-1995. doi: 10.1007/s12298-021-01066-3. Epub 2021 Sep 28.
Abscisic acid (ABA) is an important phytohormone involved in plant growth, plant development, and the protection of plants against abiotic stresses. PYL/RCAR (pyrabactin resistance/pyr1-like/regulatory components of ABA receptor) is the receptor protein of ABA and the core component of the ABA signal transduction network. The PYL gene family has been identified and analyzed in many species, however, there is no report about the research on the whole genome-wide identification of the alfalfa ( L.) PYL gene family. Therefore, to explore the function of alfalfa PYL genes, 39 MsPYL genes were identified by analyzing the recently published genome of alfalfa. Using bioinformatics methods, we systematically analyzed the chromosome location, protein physicochemical properties, evolutionary relationship, conserved motifs, and response to low-temperature stress of the MsPYL family of alfalfa. The results showed that 39 alfalfa MsPYL genes were distributed on 24 chromosomes, and the analysis of gene duplication events showed that fragment duplication was predominant duplication in alfalfa MsPYL family gene expansion. The phylogenetic tree of MsPYL protein of alfalfa and the phylogenetic tree of PYL genes of 3 species show that the MsPYL gene family can be divided into 3 subfamilies, and the structures of the same subfamilies are relatively similar. The 39 MsPYL gene family members of alfalfa contain 10 Motifs. Motif1, Motif2, Motif3, and Motif5 are the conserved motifs shared by these genes; -regulatory elements in promoter regions indicate that regulatory elements related to transcription, cell cycle, development, hormone, and stress response are abundantly present in the MsPYL promoter sequences; Real-time fluorescence quantitative PCR analysis showed that the expression of MsPYL genes can be induced by low-temperature treatment. This study provides a reference for further exploring the structural and functional characterization of the alfalfa PYL gene family.
The online version contains supplementary material available at 10.1007/s12298-021-01066-3.
脱落酸（ABA）是一种重要的植物激素，参与植物生长、发育以及植物对非生物胁迫的抵御。PYL/RCAR（抗吡唑菌素/吡1样/ABA受体调节成分）是ABA的受体蛋白，也是ABA信号转导网络的核心组成部分。许多物种中已对PYL基因家族进行了鉴定和分析，然而，关于紫花苜蓿（Medicago sativa L.）PYL基因家族全基因组鉴定的研究尚无报道。因此，为探究紫花苜蓿PYL基因的功能，通过分析最近公布的紫花苜蓿基因组鉴定出39个MsPYL基因。利用生物信息学方法，我们系统分析了紫花苜蓿MsPYL家族的染色体定位、蛋白质理化性质、进化关系、保守基序以及对低温胁迫的响应。结果表明，39个紫花苜蓿MsPYL基因分布在24条染色体上，基因复制事件分析表明片段复制是紫花苜蓿MsPYL家族基因扩增的主要复制方式。紫花苜蓿MsPYL蛋白的系统发育树以及3个物种的PYL基因系统发育树表明，MsPYL基因家族可分为3个亚家族，同一亚家族的结构相对相似。紫花苜蓿的39个MsPYL基因家族成员包含10个基序。Motif1、Motif2、Motif3和Motif5是这些基因共有的保守基序；启动子区域的调控元件表明，MsPYL启动子序列中大量存在与转录、细胞周期、发育、激素和应激反应相关的调控元件；实时荧光定量PCR分析表明，MsPYL基因的表达可被低温处理诱导。本研究为进一步探究紫花苜蓿PYL基因家族的结构和功能特性提供了参考。
在线版本包含可在10.1007/s12298-021-01066-3获取的补充材料。
