Wei Wei, Ju Jisheng, Zhang Xueli, Ling Pingjie, Luo Jin, Li Ying, Xu Wenjuan, Su Junji, Zhang Xianliang, Wang Caixiang
State Key Laboratory of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University, Lanzhou, China.
Center for Western Agricultural Research, Chinese Academy of Agricultural Sciences (CAAS), Changji, China.
Front Plant Sci. 2024 Feb 9;15:1353365. doi: 10.3389/fpls.2024.1353365. eCollection 2024.
Abiotic stress during growth readily reduces cotton crop yield. The different survival tactics of plants include the activation of numerous stress response genes, such as ().
In this study, the gene family of upland cotton was identified and analyzed by bioinformatics method, three salt-tolerant and cold-resistant genes were screened. The expression of , and in upland cotton was silenced by virus-induced gene silencing (VIGS) technique. The physiological and biochemical indexes of plants and the expression of related stress-response genes were detected before and after gene silencing. The effects of , and on salt and cold resistance of upland cotton were further verified.
We discovered 12, 6, and 6 genes in , and , respectively. Chromosomal localization indicated that the retention and loss of genes on homologous chromosomes did not have a clear preference for the subgenomes. Collinearity analysis suggested that segmental duplications were the main force for gene amplification. The upland cotton genes , and are highly expressed in roots, and is also strongly expressed in the pistil. Transcriptome data and qRT‒PCR validation showed that abiotic stress strongly induced , and . Under salt stress and low-temperature stress conditions, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and the content of soluble sugar and chlorophyll decreased in , and -silenced cotton plants compared with those in the control (TRV: 00). Moreover, -, - and -silenced cotton plants exhibited greater malondialdehyde (MDA) levels than did the control plants. Moreover, the expression of stress marker genes (, , , , , , , and ) decreased significantly in the three target genes of silenced plants following exposure to stress. These results imply that the , and genes may be regulators of salt stress and low-temperature stress responses in upland cotton.
棉花生长期间的非生物胁迫会导致作物产量大幅下降。植物的不同生存策略包括激活众多胁迫响应基因，如（）。
本研究采用生物信息学方法对陆地棉基因家族进行鉴定与分析，筛选出3个耐盐抗寒基因。通过病毒诱导基因沉默（VIGS）技术沉默陆地棉中、和基因的表达。检测基因沉默前后植株的生理生化指标及相关胁迫响应基因的表达情况。进一步验证、和对陆地棉耐盐性和抗寒性的影响。
我们分别在、和中发现了12个、6个和6个基因。染色体定位表明，同源染色体上基因的保留和丢失对亚基因组没有明显的偏好。共线性分析表明，片段重复是基因扩增的主要驱动力。陆地棉基因、和在根中高表达，在雌蕊中也强烈表达。转录组数据和qRT-PCR验证表明，非生物胁迫强烈诱导、和。在盐胁迫和低温胁迫条件下，与对照（TRV:00）相比，、和基因沉默的棉花植株中超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性以及可溶性糖和叶绿素的含量均下降。此外，、和基因沉默的棉花植株中丙二醛（MDA）含量高于对照植株。而且，在受到胁迫后，沉默植株的三个靶基因中胁迫标记基因（、、、、、、、和）的表达显著下降。这些结果表明，、和基因可能是陆地棉盐胁迫和低温胁迫响应的调节因子。
