Gong Nana, Li Fei, Wang Yanan, Li Zizi, Wang Wanqiu, Gong Tingting, Meng Xiaojun, Chen Hongtao
Department of Laboratory Medicine, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong 519000, P.R. China.
Department of Pharmacy, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong 519000, P.R. China.
Exp Ther Med. 2021 Nov;22(5):1310. doi: 10.3892/etm.2021.10745. Epub 2021 Sep 16.
Colorectal cancer ranks third in terms of incidence and second in terms of mortality worldwide. The homeobox transcript antisense intergenic RNA (HOTAIR), which was found to be located on the antisense chain of the homeobox C (HOXC) gene cluster, is a long non-coding RNA involved in multiple types of tumors. The role of HOXC11 in tumors remains unclear. Reverse transcription-quantitative PCR was performed to detect the expression level of HOXC11 in colon adenocarcinoma. Cell proliferation and invasion were assessed. RNase protection assay was used to test the possibility of RNA duplex formation. The increased expression and co-expression trend of HOXC11 and HOTAIR were identified in multiple types of cancer from The Cancer Genome Atlas and the results were validated in 12 colon adenocarcinoma and paired non-tumor tissue samples. The expression of HOXC11 and HOTAIR was found to be associated with poor prognosis in colon adenocarcinoma and kidney renal clear cell carcinoma. Furthermore, HOXC11 was found to positively regulate HOTAIR by RNA duplex formation and promoted the proliferation and invasion of colon adenocarcinoma cells.
在全球范围内，结直肠癌的发病率位居第三，死亡率位居第二。同源框转录反义基因间RNA（HOTAIR）位于同源框C（HOXC）基因簇的反义链上，是一种参与多种肿瘤的长链非编码RNA。HOXC11在肿瘤中的作用尚不清楚。进行逆转录定量PCR以检测HOXC11在结肠腺癌中的表达水平。评估细胞增殖和侵袭情况。采用核糖核酸酶保护试验检测RNA双链体形成的可能性。在癌症基因组图谱的多种癌症类型中确定了HOXC11和HOTAIR的表达增加及共表达趋势，并在12个结肠腺癌及配对的非肿瘤组织样本中验证了结果。发现HOXC11和HOTAIR的表达与结肠腺癌和肾透明细胞癌的预后不良相关。此外，发现HOXC11通过RNA双链体形成正向调节HOTAIR，并促进结肠腺癌细胞的增殖和侵袭。
