PURPOSE: Glioblastoma multiforme (GBM) is an aggressive brain tumor. This meta-analysis investigates the association between HOTAIR expression levels and GBM. METHODS: We searched the literature for studies on HOTAIR expression in GBM patients. A meta-analysis of nine studies assessed standardized mean difference (SMD) and 95% confidence intervals (CIs) using a random-effects model. Subgroup analyses were performed based on sample source, country, and study design. Additionally, we conducted meta-regression and publication bias analyses. RESULTS: The meta-analysis found a significant positive association between elevated HOTAIR expression and GBM (SMD = 8.3, 95% CI 5.8-10.8,  = 0.00). Considerable heterogeneity was observed (Q-value: 1174.2, df = 9, I = 99.2%,  = 0.00). Subgroup analyses indicated significant associations in tissue samples and studies from the USA and China. Meta-regression revealed that study design and country contributed to the observed heterogeneity, with no significant publication bias detected. CONCLUSION: This analysis confirms the significant link between HOTAIR expression and GBM, highlighting HOTAIR as a potential therapeutic target and biomarker. Further research is necessary to clarify the biological mechanisms involved in this association.