二硫苏糖醇（DTT）对IgM抗体灭活作用的评估。
Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.
作者信息
Okuno T, Kondelis N
出版信息
J Clin Pathol. 1978 Dec;31(12):1152-5. doi: 10.1136/jcp.31.12.1152.
Abstract
A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 0.0025 M and 0.005 M, pH between 7.0 and 8.0, and incubation at 37 degrees C. Concentrations over 0.01 M, pH values greater than 8.0, and an incubation temperature over 40 degrees C resulted in a gel formation of the specimen. Examination of both 'cold' and 'warm' type antibodies demonstrated that the results obtained by the DTT reagent are in close agreement with those obtained by 2-mercaptoethanol reagents and DEAE Sephadex treatment. Since the procedure is simple and rapid and lacks offensive odour, DTT is recommended for routine use in blood banking for the inactivation of IgM antibodies.
摘要
一种新引入的巯基化合物二硫苏糖醇（DTT），对其灭活IgM抗体的最佳条件进行了评估。当DTT试剂的终浓度在0.0025 M至0.005 M之间、pH值在7.0至8.0之间并在37℃孵育时，可观察到其最大效果。浓度超过0.01 M、pH值大于8.0以及孵育温度超过40℃会导致标本形成凝胶。对“冷”型和“温”型抗体的检测表明，DTT试剂得到的结果与2-巯基乙醇试剂和DEAE葡聚糖处理得到的结果非常一致。由于该方法简单快速且无刺鼻气味，因此推荐DTT用于血库中常规灭活IgM抗体。
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Vox Sang. 1974;27(5):480-8. doi: 10.1111/j.1423-0410.1974.tb02446.x.
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