Li Yinping, Tian Hao, He Qing, Geng Zhufeng, Yan Saixun, Tuniyazi Gulimeikereyi, Bai Xi
College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China.
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
ACS Omega. 2021 Sep 27;6(39):25497-25505. doi: 10.1021/acsomega.1c03420. eCollection 2021 Oct 5.
By implementing NMR, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and the UV-vis spectroscopic techniques, metabolites, mineral elements, and antioxidant activities (DPPH) of L. samples from Manasi and Fuhai of Xinjiang were studied in this research to investigate the geographical environment impact at the molecular level. First of all, partial least squares discriminant analysis was conducted to explore differential endogenous metabolites. A total of 18 metabolites were identified, and 14 mineral element contents were calculated quantitatively, which displayed diverse changing trends from these two origins. Valine, succinic acid, betaine, sucrose, and vasicine exhibited significant differences between these two groups as well as mineral nutrient profiles (Mg, Cu, N, K, Na, P, Zn, C) and DPPH antioxidant activities (EC). The obvious different characteristics of chemical components and antioxidant activities in these two groups were further verified by heat map cluster analysis. Pearson correlation analysis also revealed the remarkable relationship of chemical components and antioxidant activities, which are strongly associated with the regional environment. This study showed that the combination of methodologies proposed will be highly useful in evaluating the environmental variation and diversity in terrestrial ecosystems.
通过实施核磁共振（NMR）、电感耦合等离子体原子发射光谱法（ICP - AES）和紫外 - 可见光谱技术，本研究对来自新疆玛纳斯和福海的L.样本的代谢产物、矿物元素和抗氧化活性（DPPH）进行了研究，以在分子水平上探究地理环境的影响。首先，进行了偏最小二乘判别分析以探索差异内源性代谢产物。共鉴定出18种代谢产物，并定量计算了14种矿物元素含量，这两种来源的矿物元素含量呈现出不同的变化趋势。缬氨酸、琥珀酸、甜菜碱、蔗糖和鸭嘴花碱在这两组之间以及矿物营养成分（镁、铜、氮、钾、钠、磷、锌、碳）和DPPH抗氧化活性（EC）方面表现出显著差异。通过热图聚类分析进一步验证了这两组化学成分和抗氧化活性的明显不同特征。皮尔逊相关分析还揭示了化学成分与抗氧化活性之间的显著关系，它们与区域环境密切相关。本研究表明，所提出的方法组合在评估陆地生态系统中的环境变化和多样性方面将非常有用。
