Physiology, Exercise and Nutrition Research Group, University of Stirling.
Department of Diabetes and Endocrinology, Forth Valley Royal Hospital.
J Vis Exp. 2021 Sep 27(175). doi: 10.3791/62635.
Studies on adipose tissue are useful in understanding metabolic and other conditions. Human subcutaneous adipose tissue is accessible. With appropriate training and strict adherence to aseptic technique, subcutaneous adipose samples can be safely and efficiently obtained in a non-clinical setting by researchers. Following the administration of local anesthetic lateral to the umbilicus, a 14 G needle attached to a 5 or 10 mL syringe is inserted through the skin into the subcutaneous tissue. Under suction, the syringe is moved in a reciprocating, slicing motion to isolate fragments of adipose tissue. Withdrawing the plunger is enough to ensure that adipose tissue fragments are aspirated through the needle into the syringe. A single biopsy can collect about 200 mg of tissue. This biopsy technique is very safe for both participants and research staff. Following the biopsy, participants can resume most everyday activities, although they should avoid swimming and overly strenuous activities for 48 h to avoid excessive bleeding. Participants can safely undergo 2 biopsies within a single day, meaning that the technique can be applied in before-after acute intervention studies.
脂肪组织研究对于了解代谢和其他情况很有用。人体皮下脂肪组织是可及的。经过适当的培训并严格遵守无菌技术，研究人员可以在非临床环境中安全、有效地从皮下脂肪组织中获取样本。在局部麻醉剂注射到肚脐旁的侧方后，将一根 14G 针连接到 5 或 10 毫升的注射器上，通过皮肤插入到皮下组织。在抽吸的作用下，注射器以往复、切片的方式移动，以分离脂肪组织的碎片。拉动柱塞足以确保脂肪组织碎片通过针吸入注射器中。单次活检可以收集约 200 毫克的组织。这种活检技术对参与者和研究人员都非常安全。活检后，参与者可以恢复大多数日常活动，尽管他们应避免游泳和过度剧烈的活动 48 小时，以避免过度出血。参与者可以在同一天内安全地进行 2 次活检，这意味着该技术可以应用于急性干预研究前后。
