Chachopoulos Vasileios, Dinas Petros C, Chasioti Markella, Jamurtas Athanasios Ζ, Koutedakis Yiannis, Flouris Andreas D
FAME Laboratory, Department of Exercise Science, University of Thessaly.
FAME Laboratory, Department of Exercise Science, University of Thessaly; Institute of Sport, Faculty of Education, Health and Wellbeing, University of Wolverhampton.
J Vis Exp. 2017 Sep 30(127):55593. doi: 10.3791/55593.
Adipose tissue biopsies offer tissue samples that, upon analysis, may provide insightful overviews of mechanisms relating to metabolism and disease. To obtain subcutaneous adipose tissue biopsies in the abdominal area, researchers and physicians use either a surgical or a needle-based technique. However, surgical subcutaneous fat biopsies can offer tissue samples that may provide a more comprehensive overview of the complexities of biological indices in white adipose tissue. Usually, a surgical adipose tissue biopsy includes a diathermy treatment for cauterizing blood vessels to prevent excessive bleeding. Nevertheless, side effects, such as flash fires and skin lesions in the tissue, have been reported after diathermy. Therefore, we aimed to standardize a surgical abdominal adipose tissue biopsy performed under local anesthesia using a non-diathermy method. We conducted 115 subcutaneous adipose tissue biopsies in healthy men using a non-diathermy abdominal surgical biopsy method. Our results showed three cases of excessive post-operation bleeding out of 115 operations (2.61%).In conclusion, our standardized subcutaneous abdominal adipose tissue surgical biopsy using a non-diathermy method can be safely applied to healthy men at the bedside, with minimal side effects.
脂肪组织活检可提供组织样本，经分析后，这些样本可能会对与新陈代谢和疾病相关的机制提供有深刻见解的概述。为了获取腹部区域的皮下脂肪组织活检样本，研究人员和医生会采用手术或基于针的技术。然而，手术皮下脂肪活检所提供的组织样本可能会更全面地概述白色脂肪组织中生物指标的复杂性。通常，手术脂肪组织活检包括用电热疗法烧灼血管以防止过度出血。尽管如此，电热疗法后曾有诸如闪燃和组织皮肤损伤等副作用的报告。因此，我们旨在规范一种在局部麻醉下使用非电热疗法进行的腹部手术脂肪组织活检方法。我们采用非电热疗法腹部手术活检方法对115名健康男性进行了皮下脂肪组织活检。我们的结果显示，在115例手术中有3例术后出血过多（2.61%）。总之，我们使用非电热疗法规范的皮下腹部脂肪组织手术活检可在床边安全地应用于健康男性，且副作用最小。
