Novaes Vivian Cristina Noronha, Ervolino Edilson, Fernandes Giovani Lopes, Cunha Clara Possarle, Theodoro Leticia Helena, Garcia Valdir Gouveia, de Almeida Juliano Milanezi
Department of Diagnosis and Surgery, Division of Periodontics, School of Dentistry of Araçatuba, São Paulo State University (UNESP), St. José Bonifácio 1193 - Vila Mendonça, Araçatuba, SP, 16015-050, Brazil.
Department of Basic Science, Histology Division, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
Support Care Cancer. 2022 Mar;30(3):1967-1980. doi: 10.1007/s00520-021-06586-y. Epub 2021 Oct 11.
The determination on how antineoplastic agents interfere on the progression of periodontitis is critical for improvement and even development of novel therapeutic approaches for periodontal management. This study evaluated the influence of chemotherapy with 5-fluorouracil (5-FU) or cisplatin (CIS) on healthy periodontal tissues and on the progression of experimental periodontitis (EP).
One hundred forty-four male rats were divided into six groups (n = 24). Each group was treated with physiological saline solution (PSS) 0.9%, 5-FU, or CIS. Experimental periodontitis (EP) was induced by ligature placement. Animals were euthanized at 7, 15, and 30 days after treatment. Data were statistically analyzed (p ≤ 0.05).
The groups with EP and treated with 5-FU or CIS showed lower percentage of bone volume in the furcation region and higher percentage of alveolar bone loss, higher number of TRAP-positive cells, and lower number of PCNA-positive cells when compared group with EP and treated with PSS (p ≤ 0.05). Groups with EP and treated with 5-FU or CIS showed high immunolabelling pattern of RANKL, TNF-α, and IL-1β, moderate of BAX, and low of HIF-1α. Histological analysis showed severe tissue breakdown in the groups with EP and treated with 5-FU or CIS.
Chemotherapy with antineoplastic agents 5-FU and CIS increased the intensity and duration of the inflammation and compromised tissue repair by reduction in cellular and vascular turnover. The more severe periodontal breakdown was caused by 5-FU.
确定抗肿瘤药物如何干扰牙周炎的进展对于改进甚至开发牙周疾病管理的新治疗方法至关重要。本研究评估了5-氟尿嘧啶（5-FU）或顺铂（CIS）化疗对健康牙周组织以及实验性牙周炎（EP）进展的影响。
144只雄性大鼠分为六组（n = 24）。每组分别用0.9%生理盐水溶液（PSS）、5-FU或CIS进行处理。通过结扎诱导实验性牙周炎（EP）。在处理后7、15和30天对动物实施安乐死。对数据进行统计学分析（p≤0.05）。
与用PSS处理的EP组相比，用5-FU或CIS处理的EP组在根分叉区域的骨体积百分比更低，牙槽骨吸收百分比更高，抗酒石酸酸性磷酸酶（TRAP）阳性细胞数量更多，增殖细胞核抗原（PCNA）阳性细胞数量更少（p≤0.05）。用5-FU或CIS处理的EP组显示出核因子κB受体活化因子配体（RANKL）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的高免疫标记模式，Bax的免疫标记模式中等，低氧诱导因子-1α（HIF-1α）的免疫标记模式低。组织学分析显示，用5-FU或CIS处理的EP组出现严重的组织破坏。
使用抗肿瘤药物5-FU和CIS进行化疗会增加炎症的强度和持续时间，并通过减少细胞和血管更新来损害组织修复。5-FU导致的牙周破坏更严重。
