Oliveira Fred Lucas Pinto, Matheus Henrique Rinaldi, Ervolino Edilson, Novaes Vivian Cristina Noronha, Piovezan Bianca Rafaeli, Furquim Elisa Mara de Abreu, Fiorin Luiz Guilherme, de Almeida Juliano Milanezi
Department of Diagnosis and Surgery, Division of Periodontics, São Paulo State University "Júlio de Mesquita Filho", UNESP, Araçatuba, Brazil; Nucleus of Study and Research in Periodontics and Implantology (NEPPI), School of Dentistry, Sao Paulo, State University (Unesp), Aracatuba, SP, Brazil.
Nucleus of Study and Research in Periodontics and Implantology (NEPPI), School of Dentistry, Sao Paulo, State University (Unesp), Aracatuba, SP, Brazil; Department of Basics Sciences, Sao Paulo State University-Unesp Araçatuba School of Dentistry Sao Paulo, UNESP, Araçatuba, Brazil.
Arch Oral Biol. 2023 Nov;155:105794. doi: 10.1016/j.archoralbio.2023.105794. Epub 2023 Aug 19.
To assess sodium alendronate as a local adjunctive therapy for treating experimental periodontitis in male rats treated with chemotherapy.
One-hundred-eighty male rats were randomly divided into two groups (n = 90) based on the systemic treatments: PSS, physiological saline solution; and 5-Fluorouracil, and then, subdivided into three subgroups (n = 30): NT, no treatment; scaling and root planing; and sodium alendronate. Treatments were performed 7 days after induction of experimental periodontitis. Specimens were collected at 14, 22, and 37 days after induction. Alveolar bone level, percentage of bone in the furcation, percentage of non-vital bone in the furcation, histopathologic features, and immunolabeling pattern for tartrate-resistant acid phosphatase (TRAP) and osteocalcin (OCN) were evaluated.
The lowest amount of alveolar bone and highest amount of non-vital bone was found in group 5-Fluorouracil when no treatment was performed. In animals receiving 5-Flurouracil and subjected to periodontal treatment, adjunctive sodium alendronate resulted in higher percentage of bone in the furcation and higher alveolar bone loss, when compared with scaling and root planing alone. Better structural and cellularity patterns were found in the periodontal tissues when sodium alendronate was used, regardless of systemic treatment. Higher TRAP-expression was found when no treatment was performed. Sodium alendronate didn't affect the immunolabeling pattern of osteocalcin in the presence of 5-Fluorouracil.
Adjunctive therapy with local sodium alendronate prevented alveolar bone loss and improved the histopathological features of the periodontal tissues following scaling and root planing in male rats with experimental periodontitis receiving anticancer chemotherapy with 5-Fluorouracil.
评估阿仑膦酸钠作为局部辅助治疗手段，用于治疗接受化疗的雄性大鼠实验性牙周炎的效果。
180只雄性大鼠根据全身治疗情况随机分为两组（每组n = 90）：PSS组，即生理盐水组；5-氟尿嘧啶组，然后再细分为三个亚组（每组n = 30）：NT组，即未治疗组；龈下刮治和根面平整组；阿仑膦酸钠组。在诱导实验性牙周炎7天后进行治疗。在诱导后14天、22天和37天收集样本。评估牙槽骨水平、根分叉处骨的百分比、根分叉处无活力骨的百分比、组织病理学特征以及抗酒石酸酸性磷酸酶（TRAP）和骨钙素（OCN）的免疫标记模式。
在未进行治疗时，5-氟尿嘧啶组的牙槽骨量最低，无活力骨量最高。在接受5-氟尿嘧啶且进行牙周治疗的动物中，与单独进行龈下刮治和根面平整相比，辅助使用阿仑膦酸钠导致根分叉处骨的百分比更高，牙槽骨丧失更多。无论全身治疗情况如何，使用阿仑膦酸钠时牙周组织的结构和细胞形态模式更好。未进行治疗时TRAP表达更高。在存在5-氟尿嘧啶的情况下，阿仑膦酸钠不影响骨钙素的免疫标记模式。
在接受5-氟尿嘧啶抗癌化疗的患有实验性牙周炎的雄性大鼠中，局部使用阿仑膦酸钠进行辅助治疗可预防牙槽骨丧失，并改善龈下刮治和根面平整后牙周组织的组织病理学特征。
