Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 20025, P.R. China.
Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai 20025, People's Republic of China.
ACS Biomater Sci Eng. 2021 Nov 8;7(11):5090-5098. doi: 10.1021/acsbiomaterials.1c00815. Epub 2021 Oct 11.
Silver nanoparticles have drawn considerable attention as excellent antimicrobial agents because of their characteristics, including broad antimicrobial spectrum, durable antimicrobial property, and large specific surface area. However, the toxicity of silver nanoparticles limits the in vivo application in the antimicrobial therapy field. Here, we developed a novel silver-based biomaterial to achieve favorable biocompatibility as well as enhanced antimicrobial activity. Silver microspheres (AgMPs) were synthesized using bovine serum albumin as a template and HO as an activator. Electron microscopy results showed that AgMPs had a honeycombed inner structure with an approximate diameter of 800 nm. The minimum inhibitory concentration results exhibited that AgMPs had effective antimicrobial action against bacteria and fungi when the concentration was greater than 32 and 16 μg/mL, respectively. The cell proliferation results suggested that AgMPs have no influence on corneal epithelial cell growth when the concentration was under 25 μg/mL. The in vivo antifungal therapy experiments demonstrated that 25 μg/mL AgMPs could effectively combat wound infections. Overall, AgMPs exhibited substantial antimicrobial action on fungi in addition to biosafety on corneal epithelial cells at a concentration within 16-25 μg/mL. Our study shows that AgMPs can be used as an ocular surface drop candidate to treat fungal keratitis.
银纳米粒子因其广谱抗菌、持久抗菌和大比表面积等特性而备受关注，是一种优秀的抗菌剂。然而，银纳米粒子的毒性限制了其在抗菌治疗领域的体内应用。在这里，我们开发了一种新型的基于银的生物材料，以实现良好的生物相容性和增强的抗菌活性。使用牛血清白蛋白作为模板和 HO 作为活化剂合成了银微球（AgMPs）。电子显微镜结果表明，AgMPs 具有近似 800nm 的蜂窝状内部结构。最低抑菌浓度结果表明，AgMPs 的浓度大于 32 和 16μg/mL 时，对细菌和真菌均有有效抗菌作用。细胞增殖结果表明，AgMPs 的浓度低于 25μg/mL 时，对角膜上皮细胞的生长没有影响。体内抗真菌治疗实验表明，25μg/mL 的 AgMPs 可有效对抗 25μg/mL 的伤口感染。总体而言，AgMPs 在 16-25μg/mL 的浓度范围内对真菌具有显著的抗菌作用，同时对角膜上皮细胞具有生物安全性。我们的研究表明，AgMPs 可用作眼部表面滴剂候选物来治疗真菌性角膜炎。
