Shaul Y, Ben-Levy R, De-Medina T
EMBO J. 1986 Aug;5(8):1967-71. doi: 10.1002/j.1460-2075.1986.tb04451.x.
The hepatitis B virus (HBV) surface antigen (HBsAG) is encoded by the S gene under the regulation of a promoter in the pre-S1 region. The S gene promoter does not contain a 'TATA' box-like sequence, but there is a sequence resembling, in part, the late promoter of Simian virus 40 (SV40). In an attempt to study the regulation of the S gene promoter we looked for cellular proteins which bind to this region. We report here that a nuclear protein is tightly bound to the HBV genome at a position approximately 190 bases upstream from the S gene promoter. Evidence is provided to show that (a) this nuclear protein is the nuclear factor I (NF-I) that was previously found to be bound to the inverted terminal repeat of the adenovirus (Ad) DNA and to enhance Ad DNA replication in vitro and (b) this NF-I binding site is required for optimal activity of the S gene promoter.
乙型肝炎病毒（HBV）表面抗原（HBsAG）由S基因编码，受前S1区域启动子调控。S基因启动子不含类似“TATA”框的序列，但有一段部分类似于猿猴病毒40（SV40）晚期启动子的序列。为研究S基因启动子的调控机制，我们寻找与该区域结合的细胞蛋白。我们在此报告，一种核蛋白紧密结合于HBV基因组上，位于S基因启动子上游约190个碱基处。有证据表明：（a）这种核蛋白是先前发现与腺病毒（Ad）DNA反向末端重复序列结合并在体外增强Ad DNA复制的核因子I（NF-I）；（b）该NF-I结合位点是S基因启动子最佳活性所必需的。
