通过核型分析确定的白蛋白分离的人类精子中的性染色体比率。

Sex chromosome ratios determined by karyotypic analysis in albumin-isolated human sperm.

作者信息

Brandriff B F, Gordon L A, Haendel S, Singer S, Moore D H, Gledhill B L

出版信息

Fertil Steril. 1986 Oct;46(4):678-85.

Abstract

Human sperm that had been processed for Y-enrichment (male sex preselection) according to a currently favored albumin density gradient procedure were analyzed karyotypically for the proportion of X and Y chromosomes with the use of the human sperm/hamster egg system. This method allows direct inspection of haploid chromosome complements from human sperm. In 290 albumin-isolated sperm from six men, there were 57.2% X- and 42.8% Y-bearing chromosome complements; 201 unprocessed concurrent control sperm from five of the men had 50.2% X and 49.8% Y complements. The observed shift in sex chromosome ratio in processed samples, a decrease in Y-bearing sperm, was not statistically different from that of unprocessed controls (P = 0.13) but was significantly different when compared with the theoretic X/Y ratio of 50/50 (P = 0.016). A total of 3187 historical control karyotypes were also reviewed, with an overall sex chromosome ratio (X/Y) of 49.8/50.2. The control groups did not differ significantly from the expected 50/50. The Y-enrichment of processed sperm was not confirmed.

摘要

根据目前流行的白蛋白密度梯度法对经过Y染色体富集处理（男性性别预选）的人类精子，利用人类精子/仓鼠卵系统对X和Y染色体的比例进行了核型分析。该方法可以直接检查人类精子中的单倍体染色体组成。在来自6名男性的290个经白蛋白分离的精子中，携带X染色体的精子占57.2%，携带Y染色体的精子占42.8%；来自其中5名男性的201个未经处理的同期对照精子中，携带X染色体的精子占50.2%，携带Y染色体的精子占49.8%。在处理后的样本中观察到的性染色体比例变化，即携带Y染色体精子的减少，与未处理的对照样本相比无统计学差异（P = 0.13），但与理论上的X/Y比例50/50相比有显著差异（P = 0.016）。还回顾了总共3187个历史对照核型，总体性染色体比例（X/Y）为49.8/50.2。对照组与预期的50/50无显著差异。经处理精子的Y染色体富集未得到证实。