Luckenbach G A, Kennedy M M, Kelly A, Mandel T E
Eur J Immunol. 1978 Jan;8(1):8-12. doi: 10.1002/eji.1830080103.
We studied the function of fetal thymocytes, derived from thymus progenitor cells of 14-day-old fetuses in an organ culture system, in an in vitro humoral immune response. The unprimed cultured fetal thymocytes suppressed the T-dependent primary immune response to adult syngeneic spleen cells against sheep red blood cells, but failed to suppress the response against the T-independent antigen NIP-POL [4(hydroxy-3-iodo-5-nitrophenyl)acetyl-coupled polymerized flagellin]. The suppression was abolished by anti-Thy-1 serum and complement treatment. Using a differential killing procedure, the suppressor cells were characterized as "low" Thy-1 cells. In addition, they were resistant to hydrocortisone. Suppression could only be achieved when the cultured fetal thymocytes were added during the first 48 h of culture, suggesting that their action occurred during the induction phase of the response. The implications of the high frequency of suppressor cells during fetal life are discussed.
我们在器官培养系统中研究了源自14日龄胎儿胸腺祖细胞的胎儿胸腺细胞在体外体液免疫应答中的功能。未致敏的培养胎儿胸腺细胞抑制了针对成年同基因脾细胞对绵羊红细胞的T细胞依赖性初次免疫应答，但未能抑制针对T细胞非依赖性抗原NIP-POL[4（羟基-3-碘-5-硝基苯基）乙酰偶联聚合鞭毛蛋白]的应答。抗Thy-1血清和补体处理消除了这种抑制作用。使用差异杀伤程序，抑制细胞被鉴定为“低”Thy-1细胞。此外，它们对氢化可的松具有抗性。只有在培养的前48小时内加入培养的胎儿胸腺细胞才能实现抑制，这表明它们的作用发生在应答的诱导阶段。讨论了胎儿期抑制细胞高频率出现的意义。
