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1
Estimation of hapten-specific antibody-forming cell precursors in microcultures.微量培养中半抗原特异性抗体形成细胞前体的估计
Immunology. 1976 Feb;30(2):181-7.
2
Single cell studies on the antibody-forming potential of fractionated, hapten-specific B lymphocytes.关于分离的、半抗原特异性B淋巴细胞抗体形成潜力的单细胞研究。
Immunology. 1976 Feb;30(2):189-202.
3
Separation of antigen-specific lymphocytes. II. Enrichment of hapten-specific antibody-forming cell precursors.抗原特异性淋巴细胞的分离。II. 半抗原特异性抗体形成细胞前体的富集。
J Exp Med. 1975 May 1;141(5):1015-29. doi: 10.1084/jem.141.5.1015.
4
Antigen-initiated B-lymphocyte differentiation. VII. Quantification of AFC progenitor levels in adoptive and culture responses to NIP-POL antigen.抗原引发的B淋巴细胞分化。VII. 对NIP-POL抗原的过继性和培养反应中AFC祖细胞水平的定量分析。
Immunology. 1975 Dec;29(6):1029-40.
5
Suppression of an in vitro humoral immune response by cultured fetal thymus cells.培养的胎胸腺细胞对体外体液免疫反应的抑制作用。
Eur J Immunol. 1978 Jan;8(1):8-12. doi: 10.1002/eji.1830080103.
6
Some limits to post-antigen generation of diversity: failure to detect variants in clones of hapten-specific antibody-forming cells (AFC) developing in culture from direct AFC-progenitor B cells.抗原刺激后多样性产生的一些限制：未能在由直接AFC祖细胞B细胞体外培养产生的半抗原特异性抗体形成细胞（AFC）克隆中检测到变体。
Eur J Immunol. 1979 Aug;9(8):625-32. doi: 10.1002/eji.1830090810.
7
The potentiality of antibody-producing cells. I Bispecific cell occurrence in double stimulated cultures of syngeneic or allogeneic spleen cells of the mouse.抗体产生细胞的潜能。I. 小鼠同基因或异基因脾细胞双重刺激培养物中双特异性细胞的出现。
Immunology. 1975 Oct;29(4):653-64.
8
Plaque-forming cell responses to trinitrophenyl (TNP)-L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) in microcultures are not under conventional Ir gene control.在微量培养中，针对三硝基苯基（TNP）-L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）的噬斑形成细胞反应不受传统免疫反应基因（Ir基因）控制。
J Immunol. 1981 Oct;127(4):1534-8.
9
Role of self carriers in the immune response and tolerance. VI. Induction and reversal of TNP-modified self unresponsiveness in vitro.自身载体在免疫应答和耐受中的作用。VI. 体外诱导和逆转经三硝基苯修饰的自身无反应性
J Immunol. 1980 Dec;125(6):2380-4.
10
T cell-dependent helper and suppressive influences in an adoptive IgG antibody response.过继性IgG抗体应答中T细胞依赖性辅助和抑制作用
Immunology. 1975 Mar;28(3):485-95.

引用本文的文献

1
Requirement for persistent extracellular antigen in cultures of antigen-binding B lymphocytes.抗原结合性B淋巴细胞培养中对持续性细胞外抗原的需求。
J Exp Med. 1976 Aug 1;144(2):568-71. doi: 10.1084/jem.144.2.568.
2
Specific antibody-forming B-lymphocyte colonies. I. Distribution and nature of SRBC antibody-forming B-lymphocyte colonies in mouse lyphomyeloid organs.特异性抗体形成B淋巴细胞集落。I. 小鼠淋巴骨髓器官中抗绵羊红细胞抗体形成B淋巴细胞集落的分布与性质
Immunology. 1978 Aug;35(2):397-406.
3
Immunological T-cell memory in the in vitro-induced experimental autoimmune orchitis: specificity of the reaction and tissue distribution of the autoantigens.体外诱导实验性自身免疫性睾丸炎中的免疫T细胞记忆：反应特异性及自身抗原的组织分布
J Exp Med. 1978 Jan 1;147(1):233-50. doi: 10.1084/jem.147.1.233.
4
Role of IgD in the immune response and tolerance. I. Anti-delta pretreatment facilitates tolerance induction in adult B cells in vitro.IgD在免疫应答和耐受中的作用。I. 抗δ预处理促进成年B细胞体外耐受诱导。
J Exp Med. 1977 Dec 1;146(6):1473-83. doi: 10.1084/jem.146.6.1473.
5
Enrichment of antigen-specific B lymphocytes by the direct removal of B cells not bearing specificity for the antigen.通过直接去除对该抗原无特异性的B细胞来富集抗原特异性B淋巴细胞。
J Exp Med. 1977 Aug 1;146(2):445-56. doi: 10.1084/jem.146.2.445.
6
Tolerance induction in maturing B cells.成熟B细胞中的耐受性诱导
Immunology. 1977 Mar;32(3):283-90.
7
Functional maturation of B cells in vitro.B细胞在体外的功能成熟
Immunology. 1977 Mar;32(3):275-81.
8
Single cell studies on the antibody-forming potential of fractionated, hapten-specific B lymphocytes.关于分离的、半抗原特异性B淋巴细胞抗体形成潜力的单细胞研究。
Immunology. 1976 Feb;30(2):189-202.

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PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES.纯化抗2,4-二硝基苯基抗体的制备
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Chemical and serological studies with an iodine-containing synthetic immunological determinant 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) and related compounds.对含碘合成免疫决定簇4-羟基-3-碘-5-硝基苯乙酸（NIP）及相关化合物的化学和血清学研究。
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Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes.免疫反应中的细胞间相互作用。II. 给受照射小鼠输注骨髓、胸腺或胸导管淋巴细胞后溶血素形成细胞的来源。
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Further improvements in the plaque technique for detecting single antibody-forming cells.用于检测单个抗体形成细胞的蚀斑技术的进一步改进。
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Immunological memory in vitro.体外免疫记忆
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The in vitro response to sheep erythrocytes by mouse spleen cells: segregation of distinct events leading to antibody formation.小鼠脾细胞对绵羊红细胞的体外反应：导致抗体形成的不同事件的分离。
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微量培养中半抗原特异性抗体形成细胞前体的估计

Estimation of hapten-specific antibody-forming cell precursors in microcultures.

作者信息

Stocker J W

出版信息

Immunology. 1976 Feb;30(2):181-7.

PMID:770314
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1444979/
Abstract

The immune response of mouse spleen cells to hapten-conjugated polymer of flagellin (DNP-POL, NIP-POL) was studied using a microculture system. When increasing numbers of spleen cells were added to a 'filler' cell system, negative feedback effects became apparent and resulted in the generation of progressively lower numbers of plaque-forming cells (PFC) per input cell. This feedback inhibition was shown to be antigen-specific and mediated by factors released into the culture medium. The effect precludes calculation of the frequency of PFC precursors in cultures containing spleen cells alone and complicates the analysis of tolerance using in vitro assay systems. The addition of small numbers of spleen cells to a constant number of thymocytes provided a system in which Poisson analysis could be used to determine the frequency of PFC precursors capable of being activated by hapten-POL conjugates. This system was used to estimate the frequency of anti-NIP-PFC precursors in CBA spleen cells.

摘要

利用微量培养系统研究了小鼠脾细胞对半抗原偶联鞭毛蛋白聚合物（二硝基苯 - 聚合物，对硝基苯 - 聚合物）的免疫反应。当向“填充”细胞系统中添加越来越多的脾细胞时，负反馈效应变得明显，导致每个输入细胞产生的空斑形成细胞（PFC）数量逐渐减少。这种反馈抑制被证明是抗原特异性的，并由释放到培养基中的因子介导。这种效应使得无法单独计算含脾细胞培养物中PFC前体细胞的频率，也使使用体外测定系统分析耐受性变得复杂。向恒定数量的胸腺细胞中添加少量脾细胞提供了一个系统，在该系统中可以使用泊松分析来确定能够被半抗原 - 聚合物偶联物激活的PFC前体细胞的频率。该系统用于估计CBA脾细胞中抗对硝基苯 - PFC前体细胞的频率。