Toder V, Blank M, Nebel L
Transplantation. 1982 Jan;33(1):41-4.
The generation of active suppressor cells has been induced in vitro by preculturing of splenocytes from normal mice in the presence of mouse amniotic fluid (MAF) and alpha-fetoprotein (AFP). By using freshly explanted splenocytes, addition of such precultured lymphocytes abrogated the generation of specific cytotoxic lymphocytes (CTL) in primary mixed lymphocyte cultures. Effective suppression can be induced only when the precultured cells are added within the first 48 hr of culture. These suppressor cells are nylon-wool nonadherent and their effect could be almost abolished by treatment with anti-Thy-1,2 serum plus complement. The precursors of the suppressor cells are sensitive to hydrocortisone and resistant to low doses of radiation. The induction of suppressor cells by AFP may be one of the mechanisms resulting in deviant immune response during pregnancy and in the newborn.
通过在小鼠羊水（MAF）和甲胎蛋白（AFP）存在的情况下对正常小鼠的脾细胞进行预培养，已在体外诱导出活性抑制细胞。使用新鲜分离的脾细胞时，添加这种预培养的淋巴细胞可消除原代混合淋巴细胞培养物中特异性细胞毒性淋巴细胞（CTL）的产生。只有在培养的前48小时内添加预培养的细胞才能诱导有效的抑制作用。这些抑制细胞不黏附尼龙毛，用抗Thy-1,2血清加补体处理几乎可消除其作用。抑制细胞的前体对氢化可的松敏感，对低剂量辐射有抗性。AFP诱导抑制细胞可能是导致妊娠和新生儿期异常免疫反应的机制之一。
