Echeverría-Beirute Fabián, Varela-Benavides Ingrid, P Jiménez-Madrigal Jose, Carvajal-Chacon Milagro, Guzmán-Hernández Tomás
Instituto Tecnológico de Costa Rica, Campus Tecnológico Local San Carlos, DOCINADE, Apartado 223-21001, Alajuela, San Carlos, Ciudad Quesada, Costa Rica.
Instituto Tecnológico de Costa Rica, Campus Tecnológico Local San Carlos, Escuela de Agronomía, Apartado 223-21001, Alajuela, San Carlos, Ciudad Quesada, Costa Rica.
Biotechniques. 2021 Dec;71(6):580-586. doi: 10.2144/btn-2021-0057. Epub 2021 Oct 12.
The lack of knowledge about biological communities residing in soils, especially those in tropical regions, represents a constraint to management practices to take advantage of the ecological services provided by soil microbiota to agroecosystems. One of the complexities derived from describing biological diversity in such tropical conditions comes from the methods used to isolate microorganisms without altering the composition of the sample. The goal of this study was to establish a protocol for adequate soil sampling and environmental DNA extraction from a tropical region in Costa Rica. We present an up-to-date protocol optimized for tropical soils which improves sevenfold the amount of DNA extracted without significantly affecting the 260/280 and 260/230 ratios compared with commercially available kits and standard protocols.
对土壤中生物群落，尤其是热带地区土壤生物群落缺乏了解，这限制了利用土壤微生物群为农业生态系统提供的生态服务的管理实践。在这种热带条件下描述生物多样性所产生的复杂性之一，源于用于分离微生物而不改变样品组成的方法。本研究的目的是建立一套适用于从哥斯达黎加热带地区进行土壤采样和环境DNA提取的方案。我们提出了一种针对热带土壤优化的最新方案，与市售试剂盒和标准方案相比，该方案将提取的DNA量提高了七倍，且对260/280和260/230比值没有显著影响。
