Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias da Faculdade de Medicina (FAMED)-Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Brazil.
Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste FAMED-Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Brazil.
Mol Cell Biochem. 2022 Jan;477(1):181-189. doi: 10.1007/s11010-021-04272-5. Epub 2021 Oct 12.
The aim of the study was to investigate the in vitro and in vivo antitumor activity of leaves ethanol extract from Smilax fluminensis on murine melanoma. The extract was performed by ethylic alcohol and submitted to classical chemical analysis. Cytotoxicity test were performed on neoplastic cells, where antitumor activity was expressed in GI (concentration that inhibits 50% of cell growth) and the determination of selectivity index using a normal cell line. In addition, BALB/c mice models were used to evaluate the in vivo antitumor activity of extract in two different concentrations against B16-F10 melanoma cells. The tumor inhibition ratio was determined and the histopathological analyses of nodules and liver were compared. The chemical analysis indicated a major presence of phenolic compounds and flavonoids. Cytotoxicity test results that S. fluminensis extract was active in B16-F10 line (GI: 4.37 µg/mL), being the extract considered a promising antineoplastic agent. In the experimental model, the inhibition percentage of tumoral growth was between 78.77 and 83.49%. Histopathology analysis of nodules showed necrotic cells reduction, adipocytes presence, melanin deposition, vascularization, and inflammatory process in a concentration-dependent manner. On the liver, the animals treated with the extract on both concentrations showed normal hepatic organization, normal hepatocytes, and absence of inflammatory focus. The results indicate that S. fluminensis extract demonstrated both in vitro and in vivo antitumor activity, reducing the tumoral growth in B16-F10 and could therefore be a promising antineoplastic agent.
本研究旨在探讨菝葜叶乙醇提取物对小鼠黑色素瘤的体外和体内抗肿瘤活性。提取物采用乙醇进行提取，并进行了经典的化学分析。在肿瘤细胞上进行了细胞毒性试验，其中抗肿瘤活性以 GI（抑制 50%细胞生长的浓度）表示，并使用正常细胞系测定了选择性指数。此外，还使用 BALB/c 小鼠模型评估了提取物在两种不同浓度下对 B16-F10 黑色素瘤细胞的体内抗肿瘤活性。测定了肿瘤抑制率，并比较了结节和肝脏的组织病理学分析。化学分析表明，该提取物中主要含有酚类化合物和类黄酮。细胞毒性试验结果表明，菝葜叶提取物对 B16-F10 细胞系具有活性（GI：4.37µg/mL），因此该提取物被认为是一种有前途的抗肿瘤药物。在实验模型中，肿瘤生长的抑制率在 78.77%至 83.49%之间。结节的组织病理学分析显示，细胞坏死减少，存在脂肪细胞，黑色素沉积，血管生成和炎症过程呈浓度依赖性。在肝脏上，两种浓度下用提取物处理的动物均表现出正常的肝组织、正常的肝细胞，并且没有炎症焦点。结果表明，菝葜叶提取物在体内和体外均表现出抗肿瘤活性，降低了 B16-F10 的肿瘤生长，因此可能是一种有前途的抗肿瘤药物。
