Candida Thamyris, França Jerônimo Pereira de, Chaves Alba Lucilvânia Fonseca, Lopes Fernanda Andrade Rodrigues, Gaiba Silvana, Sacramento Celio Kersul do, Ferreira Lydia Masako, França Lucimar Pereira de
Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
Department of Agricultural and Environmental Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
Acta Cir Bras. 2014;29 Suppl 2:10-4. doi: 10.1590/s0102-86502014001400003.
To evaluate the antitumor and antimicrobial activity of ethanolic extract of Morinda citrifolia L. fruit cultivated in southeastern Brazil.
Preparation ethanolic extract of the fruit of Morinda citrifolia L. Culture of melanoma cells B16-F10 for treatment with ethanolic extract of Morinda citrifolia L. fruit to determine cell viability by MTT and determination temporal effect of ethanolic extract fruit on the cell growth B16-F10 for 8 days. Evaluation of antimicrobial activity of ethanolic extract fruit against Staphylococcus aureus and Escherichia coli by determination of Minimum Inhibitory Concentration (MIC).
The ethanolic extract of Morinda citrifolia L. fruit (10mg/mL) decreased cellular activity and inhibited 45% the rate of cell proliferation of B16-F10 melanoma treated during period studied. The ethanolic extract of Morinda citrifolia L. fruit demonstrated antimicrobial activity inhibiting the growth of both microorganisms studied. Staphylococcus aureus was less resistant to ethanolic extract of Morinda citrifolia L. fruit than Escherichia coli, 1 mg/mL and 10 mg/mL, respectively.
What these results indicate that the ethanolic extract of the fruit of Morinda citrifolia L. showed antitumor activity with inhibition of viability and growth of B16-F10 cells and also showed antibacterial activity as induced inhibition of growth of Staphylococcus aureus and Escherichia coli.
评估巴西东南部种植的巴戟天果实乙醇提取物的抗肿瘤和抗菌活性。
制备巴戟天果实乙醇提取物。培养黑色素瘤细胞B16-F10，用巴戟天果实乙醇提取物处理，通过MTT法测定细胞活力，并测定巴戟天果实乙醇提取物对B16-F10细胞生长的8天时间效应。通过测定最低抑菌浓度（MIC）评估巴戟天果实乙醇提取物对金黄色葡萄球菌和大肠杆菌的抗菌活性。
巴戟天果实乙醇提取物（10mg/mL）降低了细胞活性，并在研究期间抑制了45%的B16-F10黑色素瘤细胞增殖率。巴戟天果实乙醇提取物表现出抗菌活性，抑制了所研究的两种微生物的生长。金黄色葡萄球菌对巴戟天果实乙醇提取物的耐药性低于大肠杆菌，最低抑菌浓度分别为1mg/mL和10mg/mL。
这些结果表明，巴戟天果实乙醇提取物具有抗肿瘤活性，可抑制B16-F10细胞的活力和生长，还具有抗菌活性，可诱导抑制金黄色葡萄球菌和大肠杆菌的生长。
