电荷检测质谱法用于病毒和类病毒粒子的分析。
Charge detection mass spectrometry for the analysis of viruses and virus-like particles.
机构信息
Department of Chemistry, Indiana University, 800 E Kirkwood Ave, Bloomington 47401, Indiana.
出版信息
Essays Biochem. 2023 Mar 29;67(2):315-323. doi: 10.1042/EBC20220101.
Abstract
Heterogeneity usually restricts conventional mass spectrometry to molecular weights less than around a megadalton. As a single-particle technique, charge detection mass spectrometry (CDMS) overcomes this limitation. In CDMS, the mass-to-charge (m/z) ratio and charge are measured simultaneously for individual ions, giving a direct mass measurement for each ion. Recent applications include the analysis of viruses, virus-like particles, vaccines, heavily glycosylated proteins, and gene therapy vectors.
摘要
异质性通常将传统的质谱限制在分子量小于约 1000 道尔顿的范围内。作为一种单颗粒技术，电荷检测质谱 (CDMS) 克服了这一限制。在 CDMS 中，单个离子的质荷比 (m/z) 和电荷同时测量，从而为每个离子提供直接的质量测量。最近的应用包括病毒、类病毒颗粒、疫苗、高度糖基化蛋白质和基因治疗载体的分析。
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