Anhui Engineering Laboratory for Agro-products Processing, School of Tea & Food Science, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, Hefei 230036, China; Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
Food Chem. 2022 Mar 15;372:131213. doi: 10.1016/j.foodchem.2021.131213. Epub 2021 Sep 22.
The metabolic fate of dietary compounds is closely related to their biological functions. Pterostilbene (PT) is a methylated stilbene found in many plant foods. Herein, we investigated gastrointestinal biotransformation and tissue distribution of PT in mice fed with 0.05% PT (w/w) for 5 weeks. PT and its major metabolites i.e. PT sulfate (PT-S), pinostilbene, pinostilbene sulfate, hydroxylated PT and hydroxylated PT sulfate were identified and quantified in the mucosa and content of the digestive tissues, blood, urine and vital organs. The results showed PT underwent demethylation, hydroxylation and conjugation in the small intestine, while the conjugated metabolites were largely deconjugated in the colon. Anaerobic fermentation with mouse cecal bacteria demonstrated the microbiota mediated deconjugation and demethylation of PT-S and PT, respectively. In conclusion, oral consumption of PT led to extensive biotransformation in mouse gastrointestinal tract and the metabolites of PT might play important roles in the bioactivity of PT.
膳食化合物的代谢命运与其生物学功能密切相关。紫檀芪（PT）是一种在许多植物性食物中发现的甲基化二苯乙烯。在此，我们研究了喂食 0.05%PT（w/w）5 周的小鼠中 PT 的胃肠道生物转化和组织分布。在消化组织的黏膜和内容物、血液、尿液和重要器官中鉴定并定量了 PT 及其主要代谢物，即 PT 硫酸盐（PT-S）、松柏醇、松柏醇硫酸盐、羟基化 PT 和羟基化 PT 硫酸盐。结果表明，PT 在小肠中经历去甲基化、羟化和结合反应，而结合代谢物在结肠中主要发生去结合反应。用小鼠盲肠细菌进行的厌氧发酵表明，微生物群介导了 PT-S 和 PT 的去结合和去甲基化。总之，口服摄入 PT 导致小鼠胃肠道内广泛的生物转化，PT 的代谢物可能在 PT 的生物活性中发挥重要作用。
