Department of Pigmentation Research and Therapeutics, Graduate School of Medicine, Osaka City University, Osaka 5450051, Japan.
Biological Science Research Laboratories, Kao Corporation, Odawara 2500002, Japan.
Int J Mol Sci. 2021 Sep 27;22(19):10409. doi: 10.3390/ijms221910409.
A 308 nm monochromatic excimer light (MEL) is widely used to treat patients with vitiligo. However, dose optimization still needs to be clarified. This study aimed to obtain objective evidence regarding various doses of MEL irradiation, induced cell level changes in vitro, and skin level alterations in vivo. Cultured human keratinocytes were irradiated with MEL using various doses. After irradiation at low doses, stem cell factor, endothelin-1, and glycoprotein nonmetastatic melanoma protein B, factors that activate and protect melanocytes, were found to be significantly elevated in keratinocytes. After irradiation using medium and high doses, inflammatory cytokines were induced. The amount of ATP released and the level of inflammasome activation, which are known to be related to interleukin-1β activation, were also increased. The back skin of guinea pigs and mice were irradiated with MEL at varying doses. After irradiation, an increase of epidermal melanin and epidermal melanocytes was confirmed, using the minimal erythemal dose or less. In rhododendrol-induced leukoderma guinea pigs, a much lower dose of MEL irradiation was effective, when compared with the effective dose for control guinea pigs. Our results suggest that a lower irradiation dose of MEL might be sufficient and more suitable for repigmentation in vitiligo treatment.
308nm 单频准分子光（MEL）被广泛用于治疗白癜风患者。然而，剂量优化仍需进一步明确。本研究旨在通过体外细胞水平变化和体内皮肤水平改变，获得有关 MEL 照射各种剂量的客观证据。采用不同剂量的 MEL 对培养的人角质形成细胞进行照射。低剂量照射后，发现干细胞因子、内皮素-1 和糖蛋白非转移性黑色素瘤蛋白 B 等激活和保护黑素细胞的因子在角质形成细胞中显著升高。中、高剂量照射后，诱导产生了炎症细胞因子。已知与白细胞介素-1β激活相关的 ATP 释放量和炎症小体激活水平也增加了。采用不同剂量的 MEL 对豚鼠和小鼠的背部皮肤进行照射。照射后，采用最小红斑剂量或更低剂量，证实了表皮黑色素和表皮黑素细胞的增加。与对照组豚鼠相比，在杜鹃醇诱导的白癜风豚鼠中，MEL 照射的剂量更低，但同样有效。我们的结果表明，MEL 的低剂量照射可能足够且更适合于白癜风治疗中的复色。
