Poniatowska Aleksandra, Trzaskowska Paulina Anna, Trzaskowski Maciej, Ciach Tomasz
Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland.
Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Poleczki 19, 02-822 Warsaw, Poland.
Materials (Basel). 2021 Oct 1;14(19):5752. doi: 10.3390/ma14195752.
In this article, we present graphene oxide (produced by a modified Hummers' method) coatings obtained using two different methods: electrophoretic deposition on 316L stainless steel and chemical modification of the surface of gold applied to the steel. The coating properties were characterized by microscopic and spectrometric techniques. The contact angle was also determined, ranging from 50° to 70°. Our results indicated that GO coatings on steel and gold were not toxic towards L929 cells in a direct cell adhesion test-on all tested materials, it was possible to observe the growth of L929 cells during 48 h of culture. The lack of toxic effect on cells was also confirmed in two viability tests, XTT and MTT. For most of the tested materials, the cell viability was above 70%. They showed that the stability of the coating is the crucial factor for such GO coatings, and prove that GO in the form of coating is non-toxic; however, it can show toxicity if detached from the surface. The obtained materials also did not show any hemolytic properties, as the percentage of hemolysis was on the level of the negative control, which is very promising in the light of future potential applications.
在本文中，我们展示了采用两种不同方法获得的氧化石墨烯（通过改进的Hummers法制备）涂层：一种是在316L不锈钢上进行电泳沉积，另一种是对应用于该钢表面的金进行化学改性。通过显微镜和光谱技术对涂层性能进行了表征。还测定了接触角，其范围为50°至70°。我们的结果表明，在直接细胞粘附试验中，钢和金上的氧化石墨烯涂层对L929细胞无毒——在所有测试材料上，在48小时的培养过程中都能观察到L929细胞的生长。在XTT和MTT这两种活力测试中也证实了对细胞没有毒性作用。对于大多数测试材料，细胞活力高于70%。结果表明，涂层的稳定性是此类氧化石墨烯涂层的关键因素，并证明涂层形式的氧化石墨烯无毒；然而，如果从表面脱离，它可能会表现出毒性。所获得的材料也没有显示出任何溶血特性，因为溶血百分比处于阴性对照水平，鉴于未来的潜在应用，这是非常有前景的。
