Zako Takafumi, Matsushita Shoko, Hoshi Toru, Aoyagi Takao
Department of Materials and Applied Chemistry, Graduate School of Science and Engineering, Nihon University, 1-8-14 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan.
Materials (Basel). 2021 Oct 3;14(19):5797. doi: 10.3390/ma14195797.
In this study, the introduction of a positive charge on the surface of a shape memory material was investigated to enhance cell affinity. To achieve this, the direct chemical modification of a material surface was proposed. Sheet-type, crosslinked poly(caprolactone--α-bromo-ɤ-butyrolactone) (poly(CL--BrBL)) were prepared, and the direct reaction of amino compounds with bromo groups was conducted on the material surface with a positive charge. Branched poly(CL--BrBL) was prepared, followed by the introduction of methacryloyl groups to each chain end. Using the branched macromonomers, stable and sheet-type materials were derived through UV-light irradiation. Then, the materials were soaked in an amino compound solution to react with the bromo groups under various conditions. Differential scanning calorimetry and surface analysis of the modified materials indicated that 10 vol% of -dimethylethylenediamine in -hexane and 1 h soaking time were optimal to maintain the inherent thermal properties. The achievement of increased luminance and a positive zeta potential proved that the direct modification method effectively introduced the positive charge only on the surface, thereby enhancing cell affinity.
在本研究中，为提高细胞亲和力，对形状记忆材料表面引入正电荷进行了研究。为此，提出了对材料表面进行直接化学改性的方法。制备了片状、交联的聚（己内酯-α-溴-γ-丁内酯）（聚（CL-BrBL）），并在带正电荷的材料表面进行氨基化合物与溴基团的直接反应。制备了支化聚（CL-BrBL），随后在每个链端引入甲基丙烯酰基。使用支化大分子单体，通过紫外线照射得到稳定的片状材料。然后，将材料浸泡在氨基化合物溶液中，在不同条件下与溴基团反应。对改性材料的差示扫描量热法和表面分析表明，在正己烷中10体积%的二甲基乙二胺和1小时的浸泡时间是保持固有热性能的最佳条件。亮度增加和正ζ电位的实现证明，直接改性方法仅在表面有效地引入了正电荷，从而提高了细胞亲和力。
