Kelly R W, Deam S, Cameron M J, Seamark R F
Prostaglandins Leukot Med. 1986 Sep;24(1):1-14. doi: 10.1016/0262-1746(86)90201-5.
Antisera have been raised to the following prostaglandins as their methyl oximes; PGE2, PGD2, 13-14-dihydro-15-oxo PGE2, 13,14-dihydro-15-oxo PGF2 alpha, 6-oxo PGF1 alpha, 6-oxo PGE1 and thromboxane B2. These antisera have good specificity and sensitivity and their use allows the immediate treatment of biological fluids with oximating solution which prevents sample decomposition during storage. A methyl oximating reagent is described which gives greater than 95% conversion of PGs to their methyl oximes by treating samples at 20 degrees C overnight. The use of this reagent allows easy and reliable sample derivatisation prior to assay with the above antisera. Since the antisera (with the exception of that for thromboxane B2) do not recognise the underivatised PG or the oxime (=NOH) form, oxime formation can be carried out in parallel with methyl oxime formation and the oximated portion of the sample can act as a "reference" for the methyl oximated portion, which will allow non specific interference to be recognised.
前列腺素E2、前列腺素D2、13 - 14 - 二氢 - 15 - 氧代前列腺素E2、13,14 - 二氢 - 15 - 氧代前列腺素F2α、6 - 氧代前列腺素F1α、6 - 氧代前列腺素E1和血栓素B2。这些抗血清具有良好的特异性和敏感性，使用它们时可立即用肟化溶液处理生物体液，从而防止样品在储存期间分解。描述了一种甲基肟化试剂，通过在20℃下处理样品过夜，可使95%以上的前列腺素转化为其甲基肟。使用该试剂可在使用上述抗血清进行检测之前轻松可靠地对样品进行衍生化。由于抗血清（血栓素B2的抗血清除外）不识别未衍生化的前列腺素或肟（= NOH）形式，因此肟的形成可与甲基肟的形成同时进行，样品的肟化部分可作为甲基肟化部分的“对照”，从而识别非特异性干扰。
