Laudanski T, Kostrzewska A, Akerlund M
Prostaglandins. 1986 Jul;32(1):33-41. doi: 10.1016/0090-6980(86)90140-1.
The effect of cupric ions on the human uterus and the involvement of prostaglandins (PGs) in mediating this effect was studied by recording of isometric contractions of isolated myometrial strips and pieces of uterine arteries, and by intrauterine pressure recordings in women before the onset of menstruation. In vitro, CuCl2 in concentrations of 10(-4) M and higher caused a significant inhibition of myometrial contractile activity, but no effect on the artery preparations was seen. Furthermore, the contractile response of myometrial strips to PGF2 alpha and PGE2 (10 ng/ml) decreased in the presence of CuCl2 in concentrations of 5 and 50 mumol. In vivo, instillations of 0.3, 1.0 and 2.0 mM of CuCl2 in 0.7 ml of saline solution into the uterine cavity caused a dose-dependent stimulation of uterine activity, but after pretreatment with naproxen, 500 mg orally, the effect of these substances was abolished. After naproxen treatment, but during infusion of PGF2 alpha (5 micrograms/min), the response to the CuCl2 solutions was partially restored. It is suggested that cupric ions, at high concentrations, have an inhibiting effect on myometrial activity. The stimulatory effect of low doses of CuCl2 seen after instillation into the uterine cavity is largely exerted via initiation of synthesis and release of endometrial PGs.
通过记录离体子宫肌条和子宫动脉条的等长收缩，以及在月经来潮前对女性进行宫内压力记录，研究了铜离子对人子宫的作用以及前列腺素（PGs）在介导这种作用中的参与情况。在体外，浓度为10(-4)M及更高的CuCl2可显著抑制子宫肌层的收缩活性，但对动脉制剂未见影响。此外，在浓度为5和50μmol的CuCl2存在下，子宫肌条对PGF2α和PGE2（10ng/ml）的收缩反应降低。在体内，将0.7ml盐溶液中0.3、1.0和2.0mM的CuCl2注入子宫腔可引起子宫活动的剂量依赖性刺激，但在口服500mg萘普生预处理后，这些物质的作用被消除。在萘普生治疗后，但在输注PGF2α（5μg/min）期间，对CuCl2溶液的反应部分恢复。提示高浓度的铜离子对子宫肌层活动有抑制作用。低剂量CuCl2注入子宫腔后所见的刺激作用很大程度上是通过启动子宫内膜PGs的合成和释放来实现的。
