Zuckerman S H, Gillespie F P, Solus J F, Rybczynski R, Eisenstadt J M
Somat Cell Mol Genet. 1986 Sep;12(5):449-58. doi: 10.1007/BF01539916.
The fusion of an oligomycin (OLI)-resistant mutant of mouse LM(TK-) cells to a chloramphenicol (CAP)-resistant mutant of AK412 Chinese hamster cells resulted in a series of interspecific somatic cell hybrids. Hybrids selected in HAT medium retained only mouse mitochondrial genomes while hybrids selected in HAT plus CAP and OLI retained both hamster and mouse mitochondrial genomes in approximately equal amounts. Nuclear-coded mitochondrial proteins from both parental species were incorporated into mitochondria in all of the hybrids. However, the mitochondrially coded proteins of three individually isolated hybrid cell lines were predominantly mouse-specific, with only trace amounts of hamster protein detected.
将小鼠LM(TK-)细胞的抗寡霉素（OLI）突变体与中国仓鼠AK412细胞的抗氯霉素（CAP）突变体融合，产生了一系列种间体细胞杂种。在HAT培养基中筛选出的杂种仅保留小鼠线粒体基因组，而在HAT加CAP和OLI中筛选出的杂种保留了大致等量的仓鼠和小鼠线粒体基因组。来自两个亲本物种的核编码线粒体蛋白都被整合到所有杂种的线粒体中。然而，三个单独分离的杂种细胞系的线粒体编码蛋白主要是小鼠特异性的，仅检测到微量的仓鼠蛋白。
