Zuckerman S H, Solus J F, Gillespie F P, Eisenstadt J M
Somat Cell Mol Genet. 1984 Jan;10(1):85-91. doi: 10.1007/BF01534475.
Interspecific somatic cell hybrids were isolated following the fusion of an oligomycin-resistant derivative of LM (TK-) mouse cells to a chloramphenicol-resistant derivative of AK412 Chinese hamsters cells. Hybrids were selected in either HAT medium, HAT plus chloramphenicol (CAP), HAT plus oligomycin (OLI), or HAT plus chloramphenicol and oligomycin. Cytogenetic analysis of the hybrids indicated that their karyotype reflected the sum of the parents. Hybrids selected in HAT medium alone or HAT plus OLI retained primarily mouse mitochondrial DNA while those selected in HAT plus CAP, or HAT plus CAP plus OLI retained both species of mitochondrial DNA. There was no evidence for mitochondrial DNA recombination, despite the continued growth of these hybrids in CAP plus OLI. Hybrids that were removed from dual antibiotic selection for over three months retained both species of mitochondrial DNA in approximately equal amounts with no detectable loss or rearrangement.
将LM（TK-）小鼠细胞的耐寡霉素衍生物与AK412中国仓鼠细胞的耐氯霉素衍生物融合后，分离出种间体细胞杂种。杂种细胞在HAT培养基、HAT加氯霉素（CAP）、HAT加寡霉素（OLI）或HAT加氯霉素和寡霉素中进行筛选。对杂种细胞的细胞遗传学分析表明，它们的核型反映了亲本的总和。仅在HAT培养基或HAT加OLI中筛选出的杂种细胞主要保留小鼠线粒体DNA，而在HAT加CAP或HAT加CAP加OLI中筛选出的杂种细胞则保留两种线粒体DNA。尽管这些杂种细胞在CAP加OLI中持续生长，但没有证据表明线粒体DNA发生了重组。从双重抗生素筛选中去除三个多月的杂种细胞保留了两种线粒体DNA，且数量大致相等，没有可检测到的损失或重排。
