Ziegler M L, Davidson R L
Somatic Cell Genet. 1981 Jan;7(1):73-88. doi: 10.1007/BF01544749.
Experiments were carried out to determine whether the mitochondria-specific dye rhodamine-6G (R6G) can affect transmission of cytoplasmic determinants in mammalian cells. When one parental cell type was treated with R6G prior to fusion with an untreated partner, the subsequent hybridization frequencies in both intra- and interspecific crosses were not adversely affected, even though R6G was extremely toxic to the parental cells. In addition, cells lethally treated with R6G could be rescued by fusion with cytoplasm alone from untreated cells. When chloramphenicol (CAP) resistant cells were used as the R6G-treated parent, the expression of CAP resistance in hybrids and cybrids was greatly reduced. Thus R6G can be used to control the input of cytoplasmic determinants into fused cells. In the interspecific (Chinese hamster x mouse) crosses, it was also seen that the majority of hybrids which had not been R6G pretreated grew poorly or degenerated after a short time. In contrast, nearly all hybrids in crosses where the hamster parent was R6G pretreated grew vigorously. The concomitant elimination of inviability and loss of mitochondrial determinants in R6G-pretreated hybrids suggests that interactions involving mitochondrial gene products or components can influence growth characteristics in interspecific somatic cell hybrids.
开展了实验以确定线粒体特异性染料罗丹明-6G（R6G）是否会影响哺乳动物细胞中细胞质决定因素的传递。当一种亲本细胞类型在与未处理的伙伴细胞融合之前用R6G处理时，即使R6G对亲本细胞具有极高的毒性，种内和种间杂交随后的杂交频率也不会受到不利影响。此外，用R6G进行致死处理的细胞可以通过与未处理细胞的细胞质单独融合而得到挽救。当使用氯霉素（CAP）抗性细胞作为经R6G处理的亲本时，杂种细胞和胞质杂种细胞中CAP抗性的表达大大降低。因此，R6G可用于控制细胞质决定因素输入融合细胞。在种间（中国仓鼠×小鼠）杂交中，还观察到，大多数未经过R6G预处理的杂种细胞在短时间后生长不良或退化。相比之下，仓鼠亲本经过R6G预处理的杂交中，几乎所有杂种细胞都生长旺盛。R6G预处理杂种细胞中伴随的活力丧失和线粒体决定因素的丢失表明，涉及线粒体基因产物或成分的相互作用可以影响种间体细胞杂种细胞的生长特性。
