一种放线菌来源的具有抗炎活性的次生代谢产物。

Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria .

机构信息

Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University (KMU), Kaohsiung 807, Taiwan.

Department of Dermatology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan.

出版信息

Molecules. 2021 Sep 23;26(19):5765. doi: 10.3390/molecules26195765.

DOI:10.3390/molecules26195765

PMID:34641311

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8510368/

Abstract

Phytochemical investigation and chromatographic separation of extracts from one new actinobacteria strain that was isolated from soil of Yilan township, in the north of Taiwan, led to the isolation of nine new compounds, amycolataiwanensins A-I (-, resp.), and one , namely amycolataiwanensin J (). The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic-data analysis (1D- and 2D-NMR, MS, and UV) and comparison with literature data. The effect of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages was evaluated. Of the isolates, , , and exhibited potent anti-NO production activity, with values of 17.52, 12.31, 17.81 and 13.32 μM, respectively, compared to that of quercetin, an iNOS inhibitor with an IC value of 35.94 μM. This is the first report on indole metabolite from the genus .

摘要

从台湾北部宜兰镇土壤中分离到的一株放线菌进行植物化学研究和提取物的色谱分离，得到了 9 个新化合物，分别为 amycolataiwanensins A-I（-，依次），和一个，即 amycolataiwanensin J（）。新化合物的结构根据广泛的光谱数据分析（1D 和 2D-NMR、MS 和 UV）和与文献数据的比较，得到了明确的阐明。评价了一些分离株对脂多糖激活的 RAW 264.7 鼠巨噬细胞中一氧化氮（NO）产生抑制的影响。在所测试的分离物中，、、和表现出很强的抗 NO 产生活性，值分别为 17.52、12.31、17.81 和 13.32 μM，与 iNOS 抑制剂 quercetin 的 IC 值 35.94 μM 相比。这是首次从属中报道吲哚代谢产物。