Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego 1, 90-151 Łódź, Poland.
Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz Pomorska 141/143, 90-236 Lodz, Poland.
Biomolecules. 2020 Jan 28;10(2):194. doi: 10.3390/biom10020194.
Natural compounds isolated from plants are excellent starting points in drug design and have been widely studied as anticancer agents; they hence find use in a considerable proportion of anticancer drugs. The genus (Lamiaceae) comprises a large and widespread group of species with various applications in traditional medicine. Therefore, the aim of the present study was to determine the effectiveness of treatment with four abietane diterpenoids isolated from and , 6,7-dehydroroyleanone, 7β,6β-dihydroxyroyleanone, 7α-acetoxy-6β-hydroxyroyleanone and parvifloron D, in initiating apoptosis in a glioma cell line. The pure compounds were found to exhibit anticancer effects in primary H7PX glioma cells line by inducing apoptosis G2/M cell cycle arrest and double-strand breaks, indicated by increased levels of phosphorylated H2A.X and decreasing mitochondrial membrane potential; they also influenced the expression of pro- and anti-apoptotic genes ( or ). Our findings indicate that these compounds may offer potential as beneficial antitumor drugs but further in vivo studies are needed.
从植物中分离得到的天然化合物是药物设计的极好起点，它们已被广泛研究作为抗癌剂；因此，它们在相当一部分抗癌药物中得到应用。属（唇形科）包含一个具有各种在传统医学中的应用的大而广泛的物种群。因此，本研究的目的是确定从和分离得到的四种枞烷二萜烯的治疗效果，即 6,7-脱水罗汉松酮、7β,6β-二羟基罗汉松酮、7α-乙酰氧基-6β-羟基罗汉松酮和小蓬草 D，在诱导神经胶质瘤细胞系细胞凋亡中的作用。通过诱导 G2/M 细胞周期阻滞和双链断裂，增加磷酸化 H2A.X 的水平和降低线粒体膜电位，这些纯化合物在原发性 H7PX 神经胶质瘤细胞系中表现出抗癌作用；它们还影响促凋亡和抗凋亡基因（或）的表达。我们的研究结果表明，这些化合物可能具有作为有益的抗肿瘤药物的潜力，但需要进一步的体内研究。
