School of Advanced Science and Technology, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, 923-1292 Nomi, Ishikawa, Japan.
Chembiochem. 2021 Dec 10;22(24):3402-3405. doi: 10.1002/cbic.202100430. Epub 2021 Oct 22.
Double duplex invasion (DDI) DNA is a useful antigene method that inhibits expression of genomic DNA. We succeeded in performing photoinduced-DDI (pDDI) using ultrafast photo-cross-linking. 5-Cyanouracil ( U) has been used in pDDI to inhibit photo-cross-linking between probes, but its importance has not been clarified. Therefore, in this study, we evaluated the effect of spacer (S) and d-spacer (dS) that exhibit photo-cross-linking ability similar to that of U. U exhibited the highest pDDI efficiency, and S, dS, and T were not very different. The photo-cross-linking inhibitory effect was better with S and dS than with thymidine (T). Conversely, the thermal stability was significantly lower with S and dS than with T. The results suggest that the pDDI efficiency is determined by the balance between the photo-cross-linking inhibitory effect and the thermal stability, which is the introduction efficiency for double-stranded DNA. Therefore, U, which has a photo-cross-linking inhibitory effect and a high T value, showed the highest inhibitory efficiency.
双双链体入侵 (DDI) DNA 是一种有用的抗基因方法，可抑制基因组 DNA 的表达。我们成功地使用超快光交联进行了光诱导 DDI (pDDI)。5-氰尿嘧啶 (U) 已用于 pDDI 以抑制探针之间的光交联，但尚未阐明其重要性。因此，在这项研究中，我们评估了具有与 U 相似光交联能力的间隔物 (S) 和 d-间隔物 (dS) 的效果。U 表现出最高的 pDDI 效率，而 S、dS 和 T 则没有太大区别。S 和 dS 的光交联抑制效果优于胸腺嘧啶 (T)。相反，S 和 dS 的热稳定性明显低于 T。结果表明，pDDI 效率取决于光交联抑制效果和热稳定性之间的平衡，这是双链 DNA 的引入效率。因此，具有光交联抑制效果和高 T 值的 U 表现出最高的抑制效率。
