National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of Sciences, Shanghai 200032, China; University of Chinese Academy of Sciences, Beijing 100039, China.
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of Sciences, Shanghai 200032, China; Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Cell. 2021 Oct 28;184(22):5527-5540.e18. doi: 10.1016/j.cell.2021.09.030. Epub 2021 Oct 12.
To secure phosphorus (P) from soil, most land plants use a direct phosphate uptake pathway via root hairs and epidermis and an indirect phosphate uptake pathway via mycorrhizal symbiosis. The interaction between these two pathways is unclear. Here, we mapped a network between transcription factors and mycorrhizal symbiosis-related genes using Y1H. Intriguingly, this gene regulatory network is governed by the conserved P-sensing pathway, centered on phosphate starvation response (PHR) transcription factors. PHRs are required for mycorrhizal symbiosis and regulate symbiosis-related genes via the P1BS motif. SPX-domain proteins suppress OsPHR2-mediated induction of symbiosis-related genes and inhibit mycorrhizal infection. In contrast, plants overexpressing OsPHR2 show improved mycorrhizal infection and are partially resistant to P-mediated inhibition of symbiosis. Functional analyses of network nodes revealed co-regulation of hormonal signaling and mycorrhizal symbiosis. This network deciphers extensive regulation of mycorrhizal symbiosis by endogenous and exogenous signals and highlights co-option of the P-sensing pathway for mycorrhizal symbiosis.
为了从土壤中获取磷（P），大多数陆地植物使用通过根毛和表皮的直接磷酸盐吸收途径和通过菌根共生的间接磷酸盐吸收途径。这两种途径之间的相互作用尚不清楚。在这里，我们使用 Y1H 绘制了转录因子与菌根共生相关基因之间的网络。有趣的是，这个基因调控网络由保守的 P 感应途径控制，以磷酸盐饥饿反应（PHR）转录因子为中心。PHR 是菌根共生所必需的，通过 P1BS 基序调节共生相关基因。SPX 结构域蛋白抑制 OsPHR2 介导的共生相关基因的诱导，并抑制菌根侵染。相比之下，过表达 OsPHR2 的植物显示出改善的菌根侵染，并且对 P 介导的共生抑制有部分抗性。网络节点的功能分析揭示了激素信号和菌根共生的共同调节。该网络揭示了内源性和外源性信号对菌根共生的广泛调控，并强调了 P 感应途径被用于菌根共生的共选择。
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